An introduction to molecular biology
25 April 2017 - 28 April 2017
Organised by Caroline Pellet-Many, University College London, UK
This workshop is intended for physiologists who want to learn the basic yet essential techniques used in molecular biology. It will also illustrate the principles and methodologies behind the manipulation of protein expression using a variety of transfection techniques. 26 places are available on this course. For more information and to register, please contact Elodie Peyric
Please note that the deadline for applications is Monday 6 February 2017.
Techniques covered will include:
- Gel electrophoresis and visualisation of DNA
- Restriction enzyme digest, DNA fragment size estimation and mapping
- Bacterial transformation
- Mini‐prep (DNA extraction)
- RNA isolation and cDNA reverse transcription
- PCR (polymerase chain reaction)
- siRNA knock‐down
- EGFP analysis
- Western blot
- Protein quantification
There is no charge for this workshop, although delegates are expected to organise and fund their own accommodation.
Members of The Physiological Society are encouraged to apply for travel grant funding to support their attendance on this course.