EMBL Course: Immunoprofiling of Single Cells

04 March 2019 - 08 March 2019

EMBL Advanced Training Centre,
Meyerhofstraße 1,
Heidelberg 69117, Germany

This course will introduce participants to the cutting-edge single cell technologies provided by 10x Genomics, starting with an overview of the 10x Genomics Single Cell Controller and our associated Single Cell applications. With the release of Single Cell Feature Barcoding we are enabling analysis of protein abundance and gene expression in the same cells; for this course the focus will be immune cells. Participants will learn the key steps (and potential pitfalls) of: isolation of high-quality single cell suspensions, antibody staining, single cell library preparation, Illumina sequencing, and finally data analysis using our Loupe Cell Browser.