An introduction to molecular biology

24 April 2018 - 27 April 2018

Cruciform Building, University College London,
Gower Street,
London, United Kingdom

Organised by Caroline Pellet-Many and Laura Wisniewski, University College London, UK

This workshop is intended for physiologists who want to learn the basic yet essential techniques used in molecular biology. It will also illustrate the principles and methodologies behind the manipulation of protein expression using a variety of transfection techniques. 26 places are available on this course. For more information and to register, please contact Laura Wisniewski.

Please note that the deadline for applications is Sunday 1 April 2018.

Techniques covered will include:

  • Gel electrophoresis and visualisation of DNA
  • Restriction enzyme digest, DNA fragment size estimation and mapping
  • Bacterial transformation
  • Mini‐prep (DNA extraction)
  • RNA isolation and cDNA reverse transcription
  • PCR (polymerase chain reaction)
  • Transfection
  • siRNA knock‐down
  • EGFP analysis
  • Western blot
  • Protein quantification

There is no charge for this workshop, although delegates are expected to organise and fund their own accommodation. 

Members of The Physiological Society are encouraged to apply for travel grant funding to support their attendance on this course.