Measurement of Gene Expression using Real-Time Quantitative PCR, 19-20 April 2012

Guy's Campus, King’s College London, UK

Organised by Dr David Sugden (King’s College London) and Dr Patricia de Winter (University College London)

This two-day workshop will provide attendees with the skills required to measure mRNA levels in a sample.

The practical aspects include RNA purification and quality control for sample purity and integrity, reverse transcription, melt curve and gel analysis for specificity, and quantification of samples using a standard of known copy number with both SYBR-green and probe-based detection strategies (the latter being a new addition to this course).

Practical sessions are preceded by short explanatory lectures.  Strategies for data normalisation and relative versus absolute quantification are also covered.

Applicants must have a good understanding and some practical experience of conventional PCR (but need not have performed real-time quantitative PCR) hence this workshop would not be suitable for undergraduates.

There are 20 places available for this workshop.  To apply, please complete an application form by Wednesday 29 February 2012.

Registration is free for Members (details of how to join The Society are available here).  Registration for Non-Members is £50.  Applicants will be asked to provide payment details if they are awarded a place.

Please note the registration fee does not include accommodation, which should be arranged by the delegates.

Please email any enquiries to education@physoc.org.