Proceedings of The Physiological Society

University of Bristol (2001) J Physiol 536P, S107


Effects of antisense oligonucleotides against leech protein 4.1 upon microglial cell migration and axonal outgrowth in culture

J. Verrall and S.E. Blackshaw

Department of Human Anatomy & Genetics, University of Oxford, Oxford OX1 3QX, UK

We have developed a 3-D culture technique for analysing the function of novel genes (Blackshaw et al. 1998), isolated from a cDNA library enriched in upregulated sequences from regenerating Retzius neurons in Hirudo medicinalis (Korneev et al. 1998). We have used this culture system to assay the effects of antisense oligodeoxyribonucleotides to protein 4.1, found in this library, upon microglial cell migration and axonal outgrowth. Protein 4.1 is a structural protein, also implicated in neurotransmitter receptor targeting and RNA maturation.

Antisense 15-mer oligonucleotides against the 5

Where applicable, experiments conform with Society ethical requirements