Proceedings of The Physiological Society

Trinity College Dublin (2003) J Physiol 551P, PC38


Effect of non-selective cyclooxygenase inhibition on renal function in ischaemia-reperfusion injury in the anaesthetised normotensive and hypertensive rat

Sarah Knight and E.J. Johns

Department of Physiology, University College Cork, Eire

Prostaglandins have a regulatory role in the normal functioning of the kidney, but their involvement in the renal response to ischaemia is little understood. We investigated the effect of non-selective inhibition of cyclooxygenase (COX) enzymes on renal function up to 2.5 h after a period of ischaemia in Wistar and stroke-prone spontaneously hypertensive rats (SPSHRs).

Two groups of male Wistar and two groups of male SPSHRs (n = 5-7, 250-350 g) received polyethylene glycerol vehicle or aspirin (53.8 mg kg-1 day-1) orally for 7 days. On day 7 the rats were anaesthetised with chloralose-urethane (12 and 180 mg ml-1; 1 ml I.P. initially, 0.05 ml I.V. when necessary). A tracheostomy was performed and cannulae were placed in the right femoral vein for the infusion of inulin (1.5 g (100 ml)-1 0.9 % saline; at 3 ml h-1) and anaesthetic, and in the right femoral artery for BP, heart rate monitoring and taking plasma samples. The left ureter was cannulated for the collection of urine and the rat was left to stabilise for 1-2 h. Two baseline collections of urine and plasma were taken, and a non-traumatic clamp was placed on the renal artery for 30 min. Further urine collections were taken at 15 and 30 min, and 1, 1.5 and 2 h, and plasma samples at 0 and 30 min and 1.5 h. Animals were humanely killed with an anaesthetic overdose. Data are means and S.E.M., and were compared using ANOVA, with significance taken at P

Where applicable, experiments conform with Society ethical requirements