Proceedings of The Physiological Society

University of Cambridge (2004) J Physiol 555P, C20

Communications

A new biosensor for the measurement of extracellular ATP in the CNS

E. Llaudet, M. Droniou and N. Dale

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK


ATP is an important neurotransmitter and modulator that can act at two classes of receptors within the CNS. As many of the functions of ATP are incompletely understood, the ability to measure ATP release from the CNS in real time is a valuable tool to further understand its functions. Here we present a micro-biosensor capable of rapid in situ detection of ATP in neuronal tissue.

Building on our previous work (Llaudet et al. 2003) we have entrapped 2 enzymes, glycerol kinase and glycerol-3-phosphate oxidase, in a thin robust layer around a Pt microelectrode. The system is based on the amperometric detection of peroxide produced by the oxidation of glycerol-3-phosphate; in the first step of the enzyme cascade glycerol kinase catalyses the transfer of a phosphate from ATP to glycerol, which therefore needs to be present at concentrations of at least 500

Where applicable, experiments conform with Society ethical requirements