Proceedings of The Physiological Society
AstraZeneca (2010) Proc Physiol Soc 18, PC22
Effects of Momordica charantia fruit extract with the combination of temozolamide and cisplatin in the treatment of glioma cancer.
G. Manoharan1, R. W. Lea1, T. J. Snape1, J. Singh1
1. School of Pharmacy and Pharmaceutical Sciences, University of Central Lancashire, Preston, United Kingdom.
Prior to the availability of chemotherapeutic agents, dietary measures, including traditional medicines derived from plants were the major forms of cancer treatment (1) .One such plant is Momordic charantia (Family: Cucurbitaceae) also known as bitter gourd. M. charantia has many different chemical components that can help medicinally to treat number of diseases. Mainly, it is the green fruit part of the bitter gourd that is useful medicinally. It is composed of many different proteins and steroids that are chemically active. One such protein is α and β momorcharin which possesses anti-cancer and anti- HIV properties (2,3). This study compared the anti cancer effect of different concentrations of either the crude water and methanol soluble extract (100 µM - 400 µM) of M.Charantia, temozolamide (40 µM - 160 µM), cisplatin (50 µM - 200 µM) or α and β momocharin (200 µM - 800 µM ) on the viability of 1321N1, Gos-3, U87-MG, Weri rd1 glioma cancer cell lines compared to normal healthy L6 muscle cell line following incubation for 24 hr using 2500 cells in each 200 µl 96 well plates. The cell viability was measured using MTT assay kit for every 8 hr, 16 hr and 24 hr. Initial results have shown that either the crude water and methanol soluble extract of M.charantia, temozolamide, cisplatin or α and β Momocharin can evoke significant (P<0.05; Student‘s-t-test) decreases in cell viability for each cell line tested compared to untreated cells of each cancer cell line. These effects were dose- dependent. In another series of experiments combining either temozolamide or cisplatin with either the crude extract or α and β Momocharin had no inhibitor or additive effect on cell viability in each glioma cell line. In contrast, either the crude extract or α and β Momocharin had no significant effect on the viability of control L6 skeletal muscle cell line. In conclusion, the results have shown that both the crude extract and α and β Momocharin can elicit marked anti-cancer effects in different glioma cell lines, similar to temozolamide and cisplatin, two commercially available chemotherapeutic agents. However, a combination of either temozolamide or cisplatin failed to enhance the anti-cancer effects of either the crude extract or α and β Momocharin. Current study is focusing on the mechanisms whereby M. Charantia crude extract and α and β Momocharin can induce cell death measuring cytosolic calcium, P53, caspase- 3 activities and cytochrome c .
Where applicable, experiments conform with Society ethical requirements