Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC212
Whole Body Vibration and Antioxidant Activity
S. Lark1, D. Wadsworth1
1. School of Sport & Exercise, Massey University, Wellington, New Zealand.
In a corresponding study, the authors found that an acute bout of Whole Body Vibration (WBV) had consistently higher levels of lipid peroxidation and muscle damage compared to the metabolic equivalent exercise of walking, but less than downhill running. To combat such oxidative stress the body has two anti-oxidative systems; (a) Internal enzymes such as Glutathionine Peroxidase (GPx), and (b) Exogenous sources e.g., dietary Vitamins E and C. At times of increased oxidative stress anti-oxidant enzymes such as GPx have been found to increase in rats (1). Furthermore, training enhances the overall concentrations of antioxidant enzymes (2). Therefore this study was designed to determine changes in antioxidant activity with an acute bout of WBV exercise and any training effect in comparison to a known muscle damage inducing exercise and the metabolic equivalent exercise of walking. Twenty-one consenting untrained females (age 23.9±5 years; height 1.65±0.08m; mass 70.1±15.8 Kg) were randomised to one of three training groups: WBV (n=8; 26Hz; Galileo plate; 1min vibration/1min rest; 5mm amplitude; 20° knee flexion), Downhill Running (n=6; -10.5%, 11 km/h) or a Walking control (n=7; 4.5 km/h) - and completed 3x20min weekly supervised sessions for 8 weeks. Baseline venous blood samples were collected pre-, immediately post, and 24-h post (acute effects) and after 8 weeks (chronic effect). Quantitative colourimetric assays for Total Antioxidant Capacity (TAC) as μM Trolox equivalents and GPx activity (U/L) were compared between groups at the different measurement points via Repeated Measures ANOVA. Paired analysis between final and baseline values was performed to determine any training effects. Results are given as mean ± S.E.M. The TAC assay revealed acute increases immediate post and 24-hr post exercise for down-hill running (356μM±29, 334±22 respectively) and walking (319±12, 320±14), but not WBV (305±14, 302±11). The more specific GPx assay measuring antioxidant activity against lipid perioxidation revealed a Time main effect (p<.001). Downhill running had the highest activity for both acute and chronic responses, followed by WBV, and walking had the lowest activity (Fig 1). There was no apparent significant training effect after 8 weeks for any exercise. An acute bout of WBV does not incur greater total antioxidant activity, although WBV was consistently higher compared to the metabolic equivalent exercise of walking for the more specific GPx antioxidant activity. The same trend for the groups at different time points for GPx activity was evident, whereby downhill running increased antioxidant activity the most, then WBV and walking had the least activity. Eight weeks training may not have been long enough to up regulate activity in any of the exercise modes.
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