Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC220
Effect of hyperglycemia on perineal musculature in erectile dysfunction on streptozotocine-induced long term diabetic male rat
S. Suresh1, E. Prithiviraj1, N. Venkatalakshmi1, M. Ganesh1, S. Prakash1
1. Department of Anatomy, University of Madras, Chennai, India.
The objective of the present study is to analyze the role of perineal muscle in diabetic-induced erectile dysfunction. Male albino rat (Rattus norvegicus) were randomly divided into 2 groups (N = 12) group I (sham received citrate buffer), group II (STZ) received single i.p. injection of 60 mg/kg bw of streptozotocin (STZ) in 0.1 M citrate buffer. At the end of the experimental period (16 week) the animals were subjected to various analyses like serum hormone, erectile function, electromyogram of ischiocavernosuss (ICM) and bulbuspongiosus (BSM) (performed under Ketamine 80 mg/kg b.w., i.p. anesthesia), histology and histomorphometry of both muscle and immunohistochemistry of the synaptophysine were done. The result showed significant reduction in serum hormone (p<0.01) level, erectile function (p<0.001), reduced action potential in both muscles and wide range of histological alteration (thinning and crowding of muscle fiber) was observed in diabetic rats compared to control. Histomorphometrical study showed significant reduction in the diameter (0.001), volume (0.01) and numerical density (0.01) of the fiber in both muscles of diabetic rat compared to control. Immunolocalization of synaptophysine protein was found to be significantly (0.001) reduced in diabetic animal when compared to control. From the present study it was concluded that degenerative changes in IC and BS may play a major role in erectile dysfunction in diabetic condition. This might be due to diabetic induced postsynaptic neuronal degeneration respected with impaired motor action of the muscle. Thus while studying the treatment strategies for erectile dysfunction due consideration should be given to the integrity of the perineal musculature.
Where applicable, experiments conform with Society ethical requirements