Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC23
Post-cardiopulmonary bypass surgery acute kidney injury is associated with gene expression changes in a porcine model
M. Ghorbel1, M. Sheikh1, N. Patel1, G. D. Angelini1, G. J. Murphy1
1. Bristol Heart Institute, University of Bristol, Bristol, United Kingdom.
Acute kidney injury (AKI) is one of the most serious complications post-cardiac surgery. The pathogenesis is poorly understood. Cardiopulmonary bypass (CPB) is a major contributor to AKI. We aimed to investigate gene expression changes related to post-cardiopulmonary-bypass (CPB) AKI in a large animal model with greater homology to cardiac surgery patients. Transcriptomic analyses were performed on kidney medulla tissues obtained from pigs undergoing CPB or Sham animals. Microarray results were validated with real-time polymerase chain reaction and gene products were estimated by semi-quantitative Western blotting. Of the transcripts examined, 66 were identified as differentially expressed in CPB versus Sham pig’s kidney samples (t-Test P<0.05; 1.6 fold), with 19 (29%) up-regulated and 47 (71%) down-regulated. Only 5 and 18 out of the upregulated and downregulated transcripts respectively were annotated genes. The rest were ESTs. The regulated genes clustered into three classes Immune response, Cell adhesion/extracellular matrix and metabolic process. The upregulated genes included F5, SLC16A3 and CKMT2 whereas the downregulated genes included GST, CPE, MMP7 and SELL. Collectively, these changes indicate genetic remodelling of the kidney’s medulla in response to CPB.
Where applicable, experiments conform with Society ethical requirements