Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC288
Impact of ischemic preconditioning on excitatory postsynaptic current (EPSC) and AMPA / kainate-activated currents in primary cortical neurons
S. Maysami1,2, A. Pearson2, V. Jessick2, R. Simon2, Z. Xiong2, R. Meller2
1. Faculty of life sciences, The Unversity of Manchester, Manchester, United Kingdom. 2. Dow Neurobiology, Legacy health research, Portland, Oregon, United States.
Ischemic preconditioning is described as non-injurious ischemic stimuli that activate different signaling pathways to protect neuronal cells from a subsequent injurious ischemic insult. Rapid ischemic tolerance occurs 30 -60 min following the preconditioning event, and our recent studies suggest a synaptic mechanism may mediate the protection. Here we report our observations on primary cortical neurons from rat (Sprague-Dawley: 14 DIV) subjected to brief oxygen-glucose deprivation (30 min: ischemic preconditioning). Preconditioning significantly reduced the frequency of EPSCs recorded from cortical neurons, when measured approximately 30-45 min after 30min preconditioning (oxygen-glucose deprivation).. Ischemic preconditioning reduced AMPA and kainate-activated current amplitude and increased the desensitization time constant of AMPA-activated current. Concentration response demonstrated a shift to the right in both AMPA and kainate current amplitude. Hence, here we demonstrate for the first time that ischemic preconditioning can regulate EPSC and kinetics of AMPA / Kainate activated currents in neurons to potentially minimize the susceptibility of these cells to ischemic-injury.
Where applicable, experiments conform with Society ethical requirements