Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC337
Fenofibrate administration ameliorates the inhibitory effect of adjuvant-arthritis on leptin and adiponectin.
E. Castillero1, A. Martín1, A. Gómez-SanMiguel1, M. López-Menduiña1, M. Villanúa1, A. López-Calderón1
1. Universidad Complutense, Madrid, Spain.
Fenofibrate, a PPAR-alpha agonist prescribed to treat human dyslipidemia, has anti-inflammatory effects and improves arthritis-induced skeletal muscle wasting. Adiponectin is exclusively secreted by adipocytes and exhibits metabolic and anti-inflammatory properties. Aim: To elucidate the effect of fenofibrate administration on adiponectin and leptin secretion in arthritic rats. Methods: Arthritis was induced in male Wistar rats by an intradermal injection of 4 mg Freund’s adjuvant. Three days after the injection control and arthritis rats were divided in two experimental groups. One group was daily gavaged with 300 mg/kg bw of fenofibrate and the other was gavaged with vehicle. As arthritis decreases food intake a pair-fed group treated with vehicle was included. After 12 days of treatment rats were humanely killed, serum was collected and epididymal white adipose tissue was weighted and dissected. Adiponectin, leptin, insulin, glucose and liver glycogen were measured by commercial kits. Results: Arthritic rats had lower body and white adipose tissue weights than control and pair-fed rats (P<0.01). In arthritic rats fenofibrate administration increased body and white adipose tissue weight to similar values than those of pair-fed rats. Arthritis decreased serum concentrations of adiponectin (P<0.01) and leptin (P<0.01). Fenofibrate administration increased serum concentrations of leptin and adiponectin in arthritic but not in control rats. In contrast, fenofibrate administration did not modify arthritis-induced decrease in serum concentration of glucose (P<0.05), insulin (P0<0.01) and liver concentrations of glycogen (P<0.01). Conclusion: Fenofibrate administration increases leptin and adiponectin levels in arthritic rats. Taking into account the beneficial action of adiponectin on muscle wasting, part of the anti-cachectic action of fenofibrate can be mediated through stimulation of adiponectin secretion.
Where applicable, experiments conform with Society ethical requirements