Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC340
Galanin and different levels of energy requirements on gonadotropins levels in female goats
F. Aboutalebi1, H. Khazali1
1. Shahid Beheshti University, Tehran, Iran, Islamic Republic of.
Researchers intended to find out the underlying mechanism of the link between energy status and reproductive function.Galanin is an orexigenic agent and has been demonstrated as putative regulator of gonadotropins secretion. It is well known that orexigenic peptide probably play the agent role in the regulation of reproduction. The goal of this study was to determine the role of galanin in regulation of gonadotropins on dietry that was different in energy status. Adult female saanen goats were randomly divided into 2 groups.Animals in the first and second groups were fed 100 and 150 % energy content in diet respectively for a month. First day of the experiment, all animals were received 1 ug galanin/ kg body Weight into their jugular vein. On the second day, they were treated with 2 ug galanin/ kg body Weight. Blood samples were withdrawn from the jugular vein at 30- minute intervals, 3.5h before and 3.5h after injection by jugular veinpuncture.In order to determine the concentration of LH and FSH, plasma was isolated. RIA was applied to measure hormone concentration of gonadotropins. Two-tailed paired t test was used to compare mean plasma concentration of LH and FSH before and after of galanin injection. Results are shown as means ± SΕΜ and the significant level was set at Ρ< 0.05.The injection of 1 ug galanin in 150% dietry energy significantly reduced LH and FSH, whereas, it had no effect on the other diet. The injection of 2 ug galanin had no influence on mean concentration of gonadotropins in all two diets.Results had reflected that galanin is not considered as a main hypophyseal regulator of gonadotropins secretion in saanan goats. Infact galanin may negatively affects mean plasma concentration of LH and FSH in goats particularly with positive energy balance.
Where applicable, experiments conform with Society ethical requirements