Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC56
Multi Electrode Array recordings from the striatum and adjacent anatomical areas.
R. Smith1, E. Bracci1
1. Neuro Systems, University of Manchester, Manchester, United Kingdom.
Multi Electrode Arrays (MEAs) provide a way of recording extracellular action potentials from many tens of sites within a sample, but their use with acute slice preparations has been limited by low signal-to-noise ratios as a result of poor adherence between the slice and substrate, and by the degradation of samples due to the poor access of superperfusate to the underside of the sample. Perforated MEAs solve both of these problems by allowing negative pressure to be applied perpendicular to the recording plane of the array, providing excellent proximity between slice and electrodes and maximising the access of superperfusate to the sample. Presented here is a cheap and reliable gravity-fed negative pressure double superperfusion system for use with Multichannel Systems perforated MEAs and baseplates that easily and reliably records spikes with magnitudes of at least 4 standard deviations above noise and for durations of up to 24 hours. Using this system, spike trains were recorded from the cortex, striatum, and the globus pallidus of parasagittal slices taken from the brains of p14-p30 Sprague Dawley rats. Recordings of tonically active neurons within each region were easily obtained on multiple channels, making this technique a higher-throughput alternative to traditional methods of in vitro extracellular recording such as low impedance glass electrodes or cell-attached recording.
Where applicable, experiments conform with Society ethical requirements