Proceedings of The Physiological Society
University of Oxford (2011) Proc Physiol Soc 23, PC95
Diabetic modulation of adipose tissues CRP in piglets
E. Oclon1, J. Zubel1, K. Pierzchala-Koziec1
1. Physiology and Endocrinology, University of Agriculture in Krakow, Krakow, Poland.
Inflammation and diabetes are strongly connected with increased level of circulating cytokines. However, still little is known about the interaction between inflammation and adipose tissue. Clinical data showed that plasma levels of the proinflammatory cytokines, such as IL-6 or TNF-α changed with obesity grade and insulin resistance. Previous reports indicated that CRP (C-reactive protein) levels are correlated with body mass index (BMI) and body fat distribution. Additionally, recent studies have shown that elevation of CRP concentrations might be an independent predictive parameter of type 2 diabetes mellitus. Thus, the present study was undertaken to investigate the effect of hyperglycemia on CRP level in plasma and adipose tissues taken from piglets with induced diabetes. Studies were performed on the 8 weeks old piglets (Polish Landrace Fatteners, n=18), which were divided into control and two experimental groups. To develop hyperglycemia, animal received ip - injections of streptozotocin (150 mg/piglets over 5 days (75, 0, 50, 0 and 25mg); DM I) or glucocorticoid (60 mg/piglets over 5 days (10, 0, 20, 0 and 30mg); DM II). After completing the experiment (5 days), blood and adipose (visceral and epicardial) tissues were taken to further analysis. CRP concentrations were measured using a commercial ELISA kit (Phoenix Pharmaceuticals, USA), according to the manufacturer’s protocol. The experiment was performed according to rules accepted by The Local Ethical Commission for Investigation on Animals. The results showed that streptozotocin caused the increase of plasma CRP level from 2.51 ± 0.31 ng/ml in control group to 4.03±0.8 ng/ml in DM I (P<0.05). Unexpectedly, the significant decrease in CRP concentrations was observed in visceral adipose tissue (by 58.9%) and epicardial adipose tissue (by 32.5%) (P<0.05). There was significant increase in CRP plasma level in DM II group and similarly to piglets with DM I, significant decrease of CRP concentration was observed in visceral and epicardial adipose tissues, by 77,4% and 56,14%, respectively (P<0.05). The obtained results clearly showed that hyperglycemia modulated the CRP levels in plasma and adipose tissues. The data suggest that adipose tissues might be source of plasma CRP in diabetic piglets and that cytokines potentially play an important role in the mediating metabolic changes associated with insulin resistance and inflammation.
Where applicable, experiments conform with Society ethical requirements