Proceedings of The Physiological Society

Physiology 2012 (Edinburgh) (2012) Proc Physiol Soc 27, C89

Oral Communications

Ampicillin affects some the fatty acids and cholesterol values of rat heart tissues in non infections situation

A. Dayangac1, B. Isik1, M. Bahsi2, G. Akyildiz1, O. Yilmaz3

1. Biology, Ahi Evran University, Kirsehir, Turkey. 2. Department of Science Teacher, Firat University, Elazig, Turkey. 3. Biology, Firat University, Elazig, Turkey.


Ampicillin, a broad spectrum acid-resistant amino derivative of the penicillin group, is one of the most widely used anti-bacterial drugs in medical and veterinary medicine. In this study, we have investigated effect of ampicillin on fatty acid and cholesterol values that exist in the cell membranes and have physical significance in the Wistar albino rat heart tissues. Study were divided into two groups as control (serum physiologic) and ampicillin (25 mg/kg/day). After all experimental applications animals were anaesthetized 10 mg/kg xylazine (Rompun, Bayer, Turkey) and 70 mg/kg ketamine (Ketalar, Eczacibasi, Turkey) and animals viability were terminated by the method of taking blood from the heart under terminal anaesthesia. The fatty acids which were obtained from the heart tissues were analyzed by gas chromatography (GC) and cholesterol values were analyzed by high-performance liquid chromatography (HPLC). According to our calculations, total monounsaturated fatty acids (MUFA, such as 16:1, 18:1) values of heart tissues in the ampcillin groups were lower as statistically compared with control groups. Diametrically, total polyunsaturated fatty acids (PUFA, such as 18:2, 20:4, 22:5, 22:6) values in the same groups were higher than control group's PUFA values (p<0,05). Besides, the cholesterol values in ampicillin groups were higher than compared with control groups. According to results, we suggest that ampicillin may affect molecular structure of fatty acids as a physical in the heart tissues and may affects some fatty acids synthetase and desaturase enzyms in the liver which is center of metabolism.

Where applicable, experiments conform with Society ethical requirements