Proceedings of The Physiological Society

Obesity (2014) Proc Physiol Soc 32, PC062

Poster Communications

Behavioral and electrophysiological improvements after combined treatment with melatonin and mesenchymal stem cells in injured sciatic nerve in rats

A. Elbaz1, S. Khodir2, M. Amer2, E. Abdelmoemen1, M. Attwa1, F. Ashour3

1. Physiology, Suez Canal University, Ismailia, Ismailia, Egypt. 2. Physiology, Menofia University, Shebeen El Kom, Menofia, Egypt. 3. Physiology, Al Azhar University, Cairo, Cairo, Egypt.

Mesenchymal stem cells have some ameliorating effects in peripheral nerve injury. Melatonin also proved to be a protective agent against cellular oxidative damage. The effect of transplantation of mesenchymal stem cells in peripheral nerve injury combined with melatonin have not been tested before. The aim of our study was to evaluate this combined effect on the improvement of the injured sciatic nerve in rats.Fifty female albino rats were divided equally into 5 groups: shame control-intact peripheral nerve, control injured sciatic nerve group, injured sciatic nerve melatonin treated group(total 10mg/kg intra peritoneal divided into 2.5 mg/kg at 20 min pre, at the time, at 60 min and at 120 min post injury), injured sciatic nerve mesenchymal stem cells treated group (3x 105 cells\rat, diluted in PBS and transplanted intralesionally using insulin syringe directly after injury) and injured sciatic nerve combined melatonin and stem cells treated group with same previous doses.Behavioral assessment using the Walking Track analysis were performed in all groups once before injury and at fourth and eighth weeks after injury. Nerve conduction and EMG, using the Biopack, MP150 system were done at 8th week post injury. The measurement of total antioxidant capacity and malonaldehyde oxidase enzyme in serum were done 48 hours post injury.Mesenchymal stem cells were isolated from the human umbilical cord blood using the Ficoll-Hypaque density gradient centrifugation, then culture of mononuclear cells and selection by CD105+veCD34-veCD45-ve magnetic separation method using MACS separator.Injury to the sciatic nerve was done using the standardized crush injury method under general anesthesia by pentobarbital sodium (100 mg/kg). Complete post operative care was performed to all groups. The study was approved by the institutional ethical committee and carried out in accordance with the current guidelines for the care of lab animals.Treated groups showed significant improvement in all tested parameters compared to the injured non treated groups. The combined treatment group showed the best results of improvement in the behavioral and electrophysiological assessment.Conclusion: Treatment with combiation of mesenchymal stem cells and melatonin gives better improvement in the sciatic nerve injury than using each one of them separately.

Where applicable, experiments conform with Society ethical requirements