Proceedings of The Physiological Society

Physiology 2015 (Cardiff, UK) (2015) Proc Physiol Soc 34, PC097

Poster Communications

Maternal sleep deprivation affects morphometry and cardiovascular functions of Wistar rat offspring

Y. RAJI1

1. Department of Physiology, University of Ibadan, Ibadan, Oyo, Nigeria.


Available evidence suggests a link between altered birth morphometry and increased risk of cardiovascular disease in adulthood. Sleep deprivation during pregnancy has been associated with adverse pregnancy outcomes, but its effects on the cardiovascular function of offspring are yet to be fully elucidated. This study examined the effects of maternal sleep deprivation on birth morphometry and cardiovascular function of offspring of Wistar rats. Thirty pregnant female Wistar rats were randomly assigned to three treatment groups (n=10) from GD 1-7, 8-14 and 15-21. Half of each group (n=5) was sleep deprived (SD) on their treatment days, while the other half served as control for that group (n=5). Sleep deprivation was induced by the modified multiple platform technique1. Pup birth weight, head circumference, abdominal circumference and crown-rump length were measured at parturition. At six months postnatal life, blood pressure, heart rate, blood flow and blood volume were assessed using a computerized non-invasive blood pressure system (Kent Scientifik, USA). Data were summarized as mean ± standard error of mean and analyzed using Student's t-test and differences in means were based on 95% confidence interval and p<0.05. Systolic (144±2.7 vs. 109±3.0 mmHg, p<0.05) and diastolic (122±2.8 vs. 78±5.7 mmHg, p<0.05) pressures were higher in the GD1-7SD compared with GD1-7control. GD8-14SD had increased crown rump length (47.5±0.07 vs. 46.8±0.07 mm, p<0.05), systolic blood pressure (119±1.58 vs. 110±1.83 mmHg, p<0.05) and blood flow (34.6±4.7 vs. 14.0±1.4 mmHg, p<0.05) compared with GD8-14 control. Birth weight (5.3±0.1 vs. 5.7±0.2 g, p<0.05), systolic (76±6.3 vs. 113±3.7 mmHg, p<0.05) and diastolic (59±8.7 vs. 95±3.5 mmHg, p<0.05) pressures the GD15-21SD were reduced while crown-rump length (48.9±0.1 vs. 47.1±0.5 mm, p<0.05), was increased compared with GD15-21control. The results suggest that maternal sleep deprivation during gestation days 8-14 and 15-21 affected birth morphometry. Irrespective of the period of gestation involved, maternal sleep deprivation adversely affected elements of cardiovascular functions of offspring of Wistar rats.

Where applicable, experiments conform with Society ethical requirements