Proceedings of The Physiological Society

Physiology 2015 (Cardiff, UK) (2015) Proc Physiol Soc 34, PC107

Poster Communications

Subconvulsive dose of kainic acid transiently increases the locomotor activity of adult Wistar rats

V. Riljak1, K. Jandova1

1. Institute of Physiology, 1st Faculty of Medicine, Charles University in Prague, Czech Republic, Prague 2, Czech Republic.

Kainic acid (KA) is a potent neurotoxic substance valuable in research of temporal lobe epilepsy. We tested how subconvulsive doses of KA influence the behavior of adult Wistar rats (Rattus norvegicus). Animals were treated intraperitoneally with 5 mg/kg of KA and observed in Laboras open field test for one hour. Following behavioral parameters were quantified: time of locomotion, time of immobility, time of rearing, average speed of locomotion and distance travelled. Week after the KA treatment animals were tested again in Laboras open field test. On 8th day, animals were transcardially perfused under deep thiopental anaesthesia (40mg/kg). Rat's brains were sliced and stained with Fluoro-Jade B to detect possible neuronal degeneration. Treatment with KA increased the time spent by locomotion (p<0.01), exploratory rearing (p<0.05) and distance traveled (p<0.01), with the approximate onset thirty minutes since the KA administration. One week after the KA treatment, behavioral parameters were already the same as of the control group. Histology in terms of Fluoro-Jade B staining did not reveal any obvious neuronal damage in hippocampus. These data suggest, that subconvulsive KA dose changes the behavioral parameters only transiently. Clarification of timing of KA induced changes may help to understand mutual relationship between non-convulsive seizures and behavioral/cognitive consequences.

Where applicable, experiments conform with Society ethical requirements