Proceedings of The Physiological Society

Physiology 2016 (Dublin, Ireland) (2016) Proc Physiol Soc 37, PCA171

Poster Communications

The role of miR-378 in sarcopenia

R. McCormick1, G. Sakellariou1, A. McArdle1, K. Goljanek-Whysall1

1. Ageing and Chronic Disease, University of Liverpool, Liverpool, Please select, United Kingdom.

Sarcopenia is the loss of muscle mass and function with age and it affects all individuals from approximately the 4th decade of life. The mechanisms behind sarcopenia are unclear, however they are likely to be multifactorial. MicroRNAs (miRNAs, miRs) are small non-coding RNAs that post-transcriptionally regulate gene expression. microRNAs have been shown to be play key roles in muscle development and disease. We and others demonstrated a decrease in miR-378 expression in muscle during ageing in both rodents and humans. We manipulated the expression and function of microRNA-378 in muscle of mice using microRNA mimic and antagomiR delivered via tail vein injection. Inhibition of miR-378 expression resulted in decreased muscle mass/myofibre size and function (force generation). In contrast, overexpression of miR-378 resulted in an increased muscle mass, myofibre size and function in both adult and old mice. These data suggest miR-378 plays a role in sarcopenia and may be a potential therapeutic target against sarcopenia. Further work will focus on the validation on targets of miR-378.

Where applicable, experiments conform with Society ethical requirements