Proceedings of The Physiological Society

Physiology 2016 (Dublin, Ireland) (2016) Proc Physiol Soc 37, PCA196

Poster Communications

Correlation between nuclear envelope circularity and chromatin texture in spleen follicular cells

I. Pantic1, Y. Balcik1

1. School of Medicine, University of Belgrade, Florstadt, Germany.

It is known that various genes may influence the structure and function of nuclear envelope. However the exact relationship between chromatin organization on the larger scale, and nuclear shape remains unclear. In our study, on a sample of spleen follicular cells, we demonstrate that parameters of chromatin texture are correlated with circularity of nuclear membrane. Spleen tissue was obtained from 10 male healthy Swiss albino mice. The experiment was a part of study for which the approval had been obtained from the Ethical commission of University of Belgrade, Faculty of medicine. All procedures accorded with current national legislation/guidelines. Spleen tissue was fixated in Carnoy's solution and embedded in paraphin. Tissue sections were stained using the modification of Giemsa method for chromatin visualization as described in previously published research. A total of 200 follicular cell nuclei (20 per animal) were analyzed using National Institutes of Health (NIH, Bethesda, MD) ImageJ software and its plugins. Circularity of nuclear envelope was calculated based on the nuclear area and perimeter. Textural parameters, such as entropy (measure of textural chaos and disorder), angular second moment (indirect indicator of textural uniformity) and textural contrast were calculated using the Gray level co-occurrence matrix (GLCM) mathematical algorithm. Average entropy of chromatin was 5.12±0.18. Mean values of GLCM angular second moment and GLCM contrast were 0.051 ± 0.007 and 391.5 ± 31.1, respectively. Average circularity of the nuclear envelope was 0.93±0.01. There was a statistically highly significant (p<0.01) negative correlation between the circularity and angular second moment. No such correlation (p>0.05) was found between circularity and entropy or circularity and textural contrast. To the best of our knowledge, this is the first study to test the relationship between nuclear shape and chromatin texture in spleen follicular cells. Also, this is one of the first applications of Gray level co-occurrence matrix algorithm in chromatin structural analysis in spleen tissue. The results could be explained by physiological processes taking place in nuclear lamina that influence chromatin structure and distribution.

Where applicable, experiments conform with Society ethical requirements