Proceedings of The Physiological Society

Physiology 2016 (Dublin, Ireland) (2016) Proc Physiol Soc 37, PCA203

Poster Communications

Comparative study of piroxicam and nitroglycerin on prostaglandin modulated blood and electrolyte indices during di-oestrous in female wistar rats

E. O. Adewoye1, O. O. Adebayo1, B. O. Adele1

1. Physiology, University of Ibadan, Ibadan, Nigeria.

Background: Piroxicam and Nitroglycerin are common drugs used to treat dysmenorrhoea. Prostaglandins are involved in haemostasis, renal excretion of electrolytes and also stimulation of uterine contractions which cause dysmenorrhoea. Objective: This study compared the effects of Piroxicam and Nitroglycerin on prostaglandin mediated bleeding time, clotting time, blood concentration of sodium, potassium, urea, creatinine, and platelet count during oestrous cycle of female Wistar rats. Methods: Female Wistar rats (60) were divided into 3 groups (20 rats per group) of Control (distilled water, 0.3ml), Piroxicam treated (3mg/kg b.w) and Nitroglycerin treated (1mg/kg b.w). Animals in each group were separated into four different phases of oestrous cycle using the Pipette smear method. Treatments were administered for 4 days covering the whole length of oestrous cycle. Blood samples were collected under mild ether anaesthesia through cardiac puncture. Bleeding and clotting time were assessed and blood concentration of sodium, potassium, urea, creatinine and platelet count was evaluated. Results were expressed as Mean±S.E.M and analysed using Student t-test, level of significance was taken at p<0.05. Results: Piroxicam and Nitroglycerin caused significant increase in blood potassium (9.95±0.41 and 7.56±0.24 mmol/L) when compared to control (6.82±0.08 mmol/L) during di-oestrous. Piroxicam in addition to hyperkalemia also caused significant increase in blood urea level (5.58±0.38 vs. 4.32±0.60 mmol/L), clotting time (110±20 vs. 70±10 sec) and a significant decrease in sodium level (84.8±4.85 vs. 114.8±5.75 mmol/L) when compared to control. Nitroglycerin did not show any significant difference on all other parameters evaluated. Conclusion: Piroxicam prolonged clotting time and disrupted renal control of urea, sodium and potassium levels compared to Nitroglycerin with only hyperkalemic effects. Key Words: Piroxicam, Dysmenorrhoea, Nitroglycerin, Prostaglandins, Electrolyte.

Where applicable, experiments conform with Society ethical requirements