Proceedings of The Physiological Society

Physiology 2016 (Dublin, Ireland) (2016) Proc Physiol Soc 37, PCA216

Poster Communications

Silver nanoparticles affect regulation of testicular activity in the young rats

V. Kalynovskyi1, A. Pustovalov1, G. Grodzyuk2, N. Andrushina2, M. Dzerzhynsky1

1. Cytology, Histology and Developmental Biology Department, ESC 'Institute of Biology', Taras Shevchenko National University of Kyiv, Kyiv, Ukraine. 2. Nanomedtech-LLC, Kyiv, Ukraine.

  • Table 1. Experimental groups<\#13>

  • Table 2. Morphometric parameters of the rat testicles after the injection of silver nanoparticles<\#13>

    Mean±SEM; n=5. * - the group is significantly different from the "Control" group (p<0.05);

Silver nanoparticles (nano-Ag) are highly appreciated for their physical, chemical and antibacterial properties (Gromadzka-Ostrowska et al., 2012). But the toxicity possessed of these particles is still not well investigated (Asare et al., 2012). The effects of nano-Ag on the morphophysiology of the testis are not completely understood. That's why we investigated testicular effects of nano-Ag at different physiological conditions. The experiment was conducted on the male albino rats Rattus norvegicus (1-month-old, 130-150g, n=35). Animals were divided into 7 groups (5 rats each). The experiment lasted for 10 days. To stimulate or inhibit the reproductive system, we administrated kisspeptin-10 or peptide-234 intracerebroventricularly (I.C.V.) respectively (Qureshi & Abbas, 2013).Silver was injected in the dose of 1 mg/kg, kisspeptin-10 and peptide-234 - 3 µg/kg. Intraperitoneal injections were made on days 1-10, I.C.V. - days 8-10. To perform I.C.V. injections, animals were anesthetized with a ketamine-xylazine solution (80mg/kg + 10 mg/kg i.m.). On the last day, animals were sacrificed; their left testicle was taken and routinely histologically processed for further examination. We measured the diameter of seminiferous tubules and the cross-sectional area of Leydig cells' nuclei and Sertoli cells' nuclei. The means were compared using T-test. The obtained data is summarized in Table 2. We found that nano-Ag alone decreased Sertoli cells' activity significantly. Administration of nano-Ag blocked stimulatory effect of kisspeptin-10 while inhibitory effect of peptide-234 was mainly unaffected. This may indicate that injection of colloidal silver disrupts normal regulatory networks in the reproductive system. The exact mechanism of such effect is to be further investigated.

Where applicable, experiments conform with Society ethical requirements