Proceedings of The Physiological Society

Future Physiology (Leeds, UK) (2017) Proc Physiol Soc 39, PC04

Poster Communications

Antidiarrheal activity of hexane extract of Citrus limon peel in experimental animal model

O. S. Adeniyi1, J. Omale2, S. C. Omeje2, V. O. Edino2

1. Physiology, Benue State University, Makurdi, Benue State, Nigeria, Makurdi, Benue, Nigeria. 2. Biochemistry, Kogi State University, Anyigba, Anyigba, Nigeria.

Acute diarrhea is one of the major illnesses that cause deaths in children, despite clinical interventions and the use of Oral Rehydration Therapy (WHO, 2016). Thus, there is need to discover other effective, affordable and accessible ways to tackle this disease. Several reports have shown that Citrus limon peels have important medicinal benefits, but there are were no information about the use of this peel in the treatment of diarrhea (Sridharan et al, 2016). Therefore, this study was carried out to investigate the effects of hexane extract of Citrus limon peel (HECLP) on castor oil induced diarrhea in rats. Male albino Wistar rats weighing 100 -150g were used. Antidiarrheal activity of the extract at 5, 10 and 20mg/kg p.o was investigated by counting number of wet fecal pellets. Castor oil induced enteropooling and intestinal transit time using activated charcoal were also evaluated. Values were expressed as mean ± SEM and compared by student t-test, with n = 5 per experimental group. Result showed that the 3 doses of HECLP used significantly (p < 0.05) reduced the number of wet fecal pellets, with 20mg/kg HECLP producing the highest percentage inhibition (34.2%), however, wet fecal pellet inhibition by loperamide (3mg/kg p.o) was 68.4%, when compared with negative control. Intestinal fluid accumulation was inhibited by 68.7% and 78.5% by 20mg/kg HECLP and loperamide respectively. Furthermore, 20mg/kg HECLP significantly (p < 0.05) reduced percentage intestinal transit time (21.4 ± 1.42%), when compared with control (34.2 ± 4.29%). While atropine (5mg/kg, i.p) significantly (p < 0.001) reduced the percentage intestinal transit time to 11.2 ± 0.85%. We conclude that Citrus limon peel possess antidiarrheal effects through antisecretory and antimotility mechanisms.

Where applicable, experiments conform with Society ethical requirements