Proceedings of The Physiological Society

Future Physiology (Leeds, UK) (2017) Proc Physiol Soc 39, PC45

Poster Communications

Kolaviron modulates gastrointestinal motility and secretion of experimentally altered gut homeostasis in rats

O. A. Odukanmi1, O. E. Yelotan1, J. O. Oyekanmi1, S. B. Olaleye1

1. Department of Physiology, University of Ibadan, Ibadan, Oyo State, Nigeria.

Reports on Garcinia kola (GK) and its anti-diarrhoea effects are known but there is no report on its active component in this regard. The impact of kolaviron, an active complex of at least three compounds in extract of GK, was investigated on gut motility and secretion in experimentally altered gut homeostasis. Male Wistar rats, (189.1 ± 3.5 g) were grouped into five (n = 4/group/experiment): group 1- untreated control; group 2- treated control (positive control - animals received the gut destabilizer only in each case of experiment); groups 3, 4 and 5 received 100 mg/kg (KV100), 200 mg/kg (KV 200) kolaviron and Atropine (5 mg/kg) or Loperamide (3 mg/kg), respectively. Four different experiments were conducted to determine motility and secretion of the gut. Charcoal meal test (1 mL/ rat, p.o) assessed intestinal transit, castor oil (2 mL /animal, p.o) to establish diarrhoea and enteropooling while serotonin (1 mg/kg, i.p) was used to increase colonic motility. Determined doses of the test substances were administered for 1 hour in each case according to the groups described prior to the administration of the gut destabilizers (charcoal meal, castor oil and serotonin). Rats were anaesthetized with ketamine (90 mg/kg) and xylacine (10 mg/kg) prior to laparotomy and depending on the experiments the tissues to be examined were quickly removed except in the case of colonic motility test where animals did not require anaesthesia. Data were expressed as Mean ± SEM analyzed using one way ANOVA and considered significant at P<0.05. Kolaviron significantly decrease intestinal transit in similar way to atropine group, KV200 (27.3%), KV100 (25.7%) compared to control. Onset of diarrhea increased significantly while episodes of loose stool and purging index decreased significantly with loperamide (111.0 min, 0.4 ± 0.2, 0.1) and KV200 (128.8 min, 2.6 ± 0.7, 2.0) compared with control (52.6 min, 6.6 ± 1.0, 15.6; p<0.05), respectively. Kolaviron significantly reduced enteric fluid pooled by castor oil in KV100 (1.04 ± 0.17 mL, p<0.05) and KV200 (0.62 ± 0.21 mL, p<0.05) compared with control (1.70 ± 0.18 mL,). Colonic motility time was prolonged in KV200 (182 ±18.7 sec) compared with control (139 ± 8.72 sec). In conclusion, kolaviron exhibits potent anti-motility and anti-secretory effects on destabilized gut homeostasis and could be the major compound in Garcinia kola responsible for the previous antidiarrheal effect reported. Keywords: Motility, Secretion, Kolaviron, Garcinia kola, Diarrhoea, Enteropooling

Where applicable, experiments conform with Society ethical requirements