Proceedings of The Physiological Society

Future Physiology (Leeds, UK) (2017) Proc Physiol Soc 39, PC51

Poster Communications

Effects of soya bean supplements on blood glucose levels and some physiological parameters of alloxan induced diabetes mellitus in Wistar rats

M. N. Sada1, Y. Tanko2, A. Jimoh2, S. M. Yusuf1, O. M. Avidime1

1. Human Physiology, Faculty of Basic Medical Sciences, Kaduna State University (KASU), Kaduna, Kaduna/Tafawa balewa Way, Nigeria. 2. Human Physiology, Ahmadu Bello University, Zaria, Zaria, Kaduna, Nigeria.

Introduction: Uncontrolled diabetes leads to several complications that affect many organs of the body. Diet plays an important role in the management of diabetes mellitus and the health- beneficial effects of dietary fibres and antioxidants derived from plant food sources including soya beans are being extensively studied. Aim: The aim of this study was to evaluate the effects of soya bean supplements on blood glucose levels and some physiological parameters of Alloxan-induced diabetes mellitus in Wistar rats. Methodology: Twenty albino Wistar rats of both sexes weighed between 120-150 grams were used for the study. Induction of diabetes was done by a single intraperitoneal injection of Alloxan Monohydrate at a dose of 150mg/kg body weight (Katsumata et al., 1999). Rats having fasting blood glucose levels of 200mg/dL and above after the induction period were used for the study. The diabetes induced animals were grouped into four groups of five rats each: Group 1 (negative control) received distilled water orally for two weeks; Group 2 (positive control) were administered 5mg/kg body weight of glibenclamide orally for two weeks; Groups 3 and 4 were fed with 25% and 50% soya bean supplements respectively for two weeks. The fasting blood glucose levels were determined at intervals of 0, 1, 3, 6, 9 and 12 days respectively using the glucose oxidase method (Beach and Turner, 1958) with the aid of a digital glucometer. At the end of the two weeks experiment, the animals were anaesthetised at the time of sacrifice by being placed in a sealed cotton wool soaked chloroform inhalation jar. Blood samples were taken from all the groups for the determination of lipid profile, liver enzymes and haematological parameters. Data obtained were analysed using analysis of variance (ANOVA). Results: After two weeks of supplementation with soya bean in the animal diet, the fasting blood glucose levels, lipid profile and liver enzymes were significantly reduced (P ≤ 0.05) in the soya beans group as compared with the control group. As regards the haematological parameters, there was no significant difference between the control and soya bean supplemented group. Conclusion: Soya bean supplements were found to have blood-glucose lowering potential, anti-lipidaemic and improved liver enzymes activity in Alloxan-induced diabetic Wistar rats.

Where applicable, experiments conform with Society ethical requirements