Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, C056

Oral Communications

Effect of time-restricted feeding and exercise on metabolic diseases in mice

G. Raza1, V. Stenbäck1, Y. Kaya1, S. Mutt1, K. A. Mäkelä1, K. Herzig1,2,3

1. Research unit of Biomedicine, University of Oulu, Oulu, Please select an option below, Finland. 2. Medical Research Center Oulu, University of Oulu, Oulu, Finland. 3. Gastroenterology, Poznan University of Medical Sciences, Poznan, Poland.

Background: Obesity is a major global threat and risk factor for type-2 diabetes, hypertension, cardiovascular diseases and cancer. Diet and exercise are the two major regulators of adiposity. In addition time-restricted feeding has been shown to improve clock and nutrient sensor functions. Method: To test whether high fat diet (HFD) with or without exercise results in decreased metabolic disruption and weight gain ,we fed male mice (C57BL6) either time-restricted (t-RF) or ad libitum with HFD (60% Kcal fat) or control diet (CD, 10% Kcal Fat) for 12 weeks. Zeitgeber time 0 (ZT0) was designated as lights-on time and ZT12 as lights-off time. The t-RF mice were allowed to eat for 10 hours between ZT15-ZT24. HFD (t-RF and ad libitum) fed mice were exercised on a treadmill with a speed of 12m/min for 1 hour every other day. Body weight and food intake were measured every second day. Insulin and glucose tolerance tests were performed after 8 and 12 weeks of feeding. Fasting plasma HDL, LDL, total cholesterol and triglycerides were measured after 12 weeks of feeding. The differences between the groups were analyzed using one-way ANOVA for significance. Results: HFD feeding (t-RF or ad libitum) significantly (p<0.0067) increased body weight gain compared to CD fed mice. The t-RF significantly decreased body weight gain by 51% in HFD, while no significant changes were observed in CD fed mice. In addition, exercise significantly decreased body weight gain by 34% in ad libitum fed mice, while no effects were seen in t-RF mice. The t-RF mice on HFD significantly (p<0.019) decreased food intake compared with ad libitum fed. Exercise did not change food intake either on t-RF or ad libitum fed compared to their respective controls. HFD feeding significantly increased plasma LDL-Cholesterol and total cholesterol compared to CD. Time-restriction significantly (p<0.0005) lowered plasma LDL-cholesterol and total cholesterol in HFD fed, but not in CD fed mice. Furthermore, exercise significantly (p<0.03) increased plasma HDL- Cholesterol in ad libitum fed mice. The t-RF significantly improved glucose tolerance in HFD and CD fed mice (p<0.05). In addition, exercise significantly (p<0.001) improved insulin sensitivity in t-RF fed mice, while it had no effect on ad libitum fed mice. Conclusion: Time-restricted feeding significantly improves the metabolic profiles and reduces adiposity in HFD induced obese mice. Exercise increases the plasma HDL and hence cardio metabolic fitness. Time-restricted feeding in combination with exercise improves metabolic profiles and might decrease obesity and metabolic diseases.

Where applicable, experiments conform with Society ethical requirements