Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, C090

Oral Communications

Effects of crocins on intacerebroventricular streptozotocin induced neuroinflammation and peripheral immune changes in experimental rats of Alzheimer's disease

R. Ghosh1

1. DEPARTMENT OF PHYSIOLOGY, University of Calcutta, Kolkata, India.

Different animal models are used to investigate the mechanism of neurodegeneration in AD. Intracerebroventricular streptozotocin injected experimental rats of Alzheimer disease (ICV STZ-AD) develop insulin resistant brain state associated with memory impairment and neurodegenerative changes that share many features in common with sporadic Alzheimer disease (AD) in humans. A possible neuroinflammatory mechanism has been implicated in the memory impairment and neurodegeneration in ICV STZ-induced experimental AD rats which might influence the peripheral immune compartment through the blood brain barrier. The antioxidative and anti-inflammatory effects of crocins have been studied in different experimental animal models of cerebral ischemia, acute and chronic inflammation. The anti-inflammatory and antioxidative potentials of crocins have not been sufficiently explored in the ICV STZ-AD rats. Crocin was applied to find any protective role on the ICV STZ induced neuroinflammation( proinflammatory markers namely TNF-α and IL-1β, ROS and nitrite), histopathological( Cresyl violet staining for Nissl granules) and peripheral immune changes. Brain levels of TNF-α and IL-1β, ROS and nitrite levels were significantly increased in hippocampus and serum of ICV STZ AD rats. The appreciable changes in most of the inflammatory parameters and chromatolysis of Nissl granules of STZ- AD rats were protected after i.p. administration of crocin in these rats. Thus it can be concluded that Crocins by its antioxidative and anti-inflammatory processes can inhibit the STZ induced neuroinflammation and peripheral immune changes in AD rats.

Where applicable, experiments conform with Society ethical requirements