Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA005

Poster Communications

Effects of levosimendan in rats with lipopolysaccharide-induced disseminated intravascular coagulation

H. Tsai3, S. Chen2, C. Wang1, C. Tsao4, W. Liaw5, C. Wu1

1. Pharmacology, National Defense Medical Center, Taipei, Taiwan. 2. Department of Long-Term Care, College of Nursing and Health, Kang-Ning University, Taipei, Taiwan. 3. Department of Anesthesiology, Mackay Memorial Hospital, Taipei, Taiwan. 4. Department of Anesthesiology, Taipei Veterans General Hospital, Taipei, Taiwan. 5. Department of Anesthesiology, Tungs' Taichung MetroHarbor Hospital, Taipei, Taiwan.

Disseminated intravascular coagulation (DIC) is a common complication of sepsis and leads to multiple organ failure and death. In patients with severe sepsis, pro-inflammatory cytokines and activation of tissue factors trigger a cascade of events leading to coagulation dysfunction and multiple organ failure (MOF). Levosimendan (LS) has been demonstrated that it has protective effects against tissue injury caused by endotoxin. The aim of this study was to examine effects of levosimendan on consumptive coagulopathy and organ dysfunction in an endotoxaemic animal model induced by lipopolysaccharide (LPS). Here, we tried to investigate effects of levosimendan on haemostatic changes in LPS-induced DIC by using Thromboelastography (TEG). Male adult Wistar rats were randomly divided into four groups: Control group, an intravenous infusion of 5% dextrose 1.2 mL/kg for 20 minutes (24 ug/kg for 20 minutes plus 0.6 ug/kg/min for 4 hours); LPS group, an intravenous LPS (4 mg/kg) infusion followed by dextrose administration; and LS-treated LPS group, an intravenous LPS infusion followed by LS treatment. Haemodynamics, biochemistry, haemostasis and inflammatory response were examined during the experimental period. The administration of LS significantly attenuated (i) consumptive coagulopathy displayed by TEG, (ii) the decreases of platelet count and plasma interleukin-6 in rats treated with LPS. Thus, the treatment of LPS rats with LS reduced organ injury and coagulopathy and this protective effect may be attributed to its anti-inflammatory effects.

Where applicable, experiments conform with Society ethical requirements