Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA008

Poster Communications

Actions of aqueous extract of roots of Carissa edulis (Natal Plum) on cardiac properties of isolated rabbit's heart.

M. YUSUF1, M. A. Mabrouk2, K. A. MUHAMMED1

1. Human Physiology, Kaduna State University., Kaduna, Kaduna, Nigeria. 2. Human Physiology, Bayero University, Kano, Kano, Nigeria.


Hypertension is one of the major risk factors in both coronary heart disease and cerebrovascular accidents; it often remains asymptomatic until late in its course. Carissa edulis Vahl is a shrubby plant called Natal Plum in English. It is predominant in the northern region of Nigeria. The plant have been claimed to treat numerous ailments including hypertension. This study was designed to investigate the effects of the aqueous extract of the root of Carissa edulis on cardiac properties of a rabbit's heart ex vivo, using the Langendorff apparatus and technique. The apparently healthy and non-treated research animal was sacrificed by cervical dislocation, the heart was accessed after dissection of the thoracic cavity and mounted on the apparatus, upon which the test extract and other standard drugs were applied for their various effects. Especially for their chronotropic and inotropic effects, which were recorded by a Physiograph on a tracing paper. Data obtained were statistical analyzed using the paired student's t-test and the results indicated that aqueous extract of root of Carissa edulis showed a dose dependent effects in causing a negative chronotropic and inotropic effects on the isolated rabbit's heart. The maximum effect was observed on administration of 3.0mg of the extract, which caused a decrease in the heart rate from 120 beats/min to 90 beats/min (p < 0.05) and a decrease in strength of contraction from 7.92 ± 0.154 mV to 1.50 ± 0.129 mV (p < 0.05). The extract was seen to exhibit a similar effect as Acetylcholine on the organ, and was antagonized by Atropine. We therefore conclude that aqueous root extract of Carissa edulis has a negative chronotropic and inotropic effects on an isolated heart of a rabbit and may possess the antihypertensive properties.

Where applicable, experiments conform with Society ethical requirements