Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA086

Poster Communications

Microelectrode Techniques for Cell Physiology - The Plymouth Workshop 2018.

D. ogden1

1. Marine Biological Association, Plymouth, United Kingdom.

The ‘Microelectrode Techniques Workshop' 2018 is the 35th edition of the course hosted every September by the Marine Biological Association at its Plymouth Laboratory. The course teaches hands-on technical aspects of electrophysiological and optical recording in single cells and tissues to 20 students, mainly post grad with around 4 post-docs. Several variations of voltage clamp, patch clamp and voltage recording methods are used to highlight the common technical problems encountered. A day is spent on circuit design, construction and testing, and demonstrations of signal processing and sampling. For optical recording, the microscope is reduced to optical elements and their properties explained, and fluorescent indicators and labels are used in conjunction with electrophysiological recording. There are 25 lectures in basic and more specialised recording techniques. The hands-on experiments are taught as 3-day practicals in voltage or optical recording, including ion selective electrodes, voltage clamp, and patch clamp single cell or slice techniques. Specialised demonstrations and experiments are given for capacitance, amperometry, dynamic clamp, perforated patch and multi-electrode arrays. Optical methods taught are Ca or pH fluorescent indicator imaging with cameras, TIRF microscopy, microelectrode injection and virus fluorescent protein cell labelling techniques, and photolysis. There is a hands-on optical bench to demonstrate combining of optical components with light sources and detectors. Optogenetic methods are demonstrated and used in experiments with ChR in C.elegans and at the Drosophila neuromuscular junction. The tuition for hands-on is one on one; this has proved to be the best way to learn the technical constraints associated with each method. There are around 45 instructors and lecturers who teach the course each year, some from instrument companies who loan much of the instrumentation. The poster will illustrate the course in 2018. , Twitter: @CPW_plymouth

Where applicable, experiments conform with Society ethical requirements