Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA090

Poster Communications

Is Notch -4 Associated with VEGF-A and IL-6 in the CRC Tumorigenesis?

N. erkasap1, R. ÖZYURT1, M. OZKURT1, S. ERKASAP2, N. YASAR2, E. IHTIYAR2, F. CANAZ3, E. YILMAZ3, E. ÇOLAK4

1. PHYSIOLOGY, ESKISEHIR OSMANGAZI UNIVERSITY MEDICAL FACULTY, Eskisehir, Turkey. 2. GENERAL SURGERY, ESKISEHIR OSMANGAZI UNIVERSITY, Eskisehir, Turkey. 3. PATHOLOGY, ESKISEHIR OSMANGAZI UNIVERSITY, Eskisehir, Turkey. 4. STATISTICS, ESKISEHIR OSMANGAZI UNIVERSITY, Eskisehir, Turkey.


Colorectal cancer (CRC) is the third most common cancer in the world wide, the morbidity of patients with CRC has been increasing fastly in recent years. Recent studies have indicated that the Notch signaling plays an important role in the development and progression of cancer. The Notch signaling pathway includes Notch receptors and Notch ligands. These ligands and receptors of the Notch pathway are known to be highly oncogenic in CRC, but the effect of Notch-4 is still not clear. Angiogenic and pro-inflammatory cytokines such as VEGF and IL (interleukin)-6 are involved in CRC development mechanisms. However, other regulatory mechanisms such as Notch ligands and receptors are parallel to VEGF-A and IL-6 mediated cellular changes. The role of these three molecules has not yet been fully understood in the CRC. The aim of our study was to analyze the expression of Notch4, IL-6 and VEGF-A in malignant and neighboring normal colorectal tissues. This study was conducted with the approval of the Human Ethics Committee of Eskisehir Osmangazi University, Turkey in accordance with the Decleration of Helsinki (Approval No. 80558721/90). A total of 40 patients with CRC who underwent surgical resection at the Department of General Surgery, Eskisehir Osmangazi University Hospital were analyzed in this study. Notch4, IL-6 and VEGF-A expression were detected by using real-time polymerase chain reaction in tumorous (n:40) and neighboring normal (n:40) colorectal tissues. According to RT-PCR results, in tumorous tissue comparison with normal tissues, mRNA levels of Notch4, VEGF-A and IL-6 increased respectively (p<0.05), (p<0.001) and (p<0.001). Our study suggest that play a key role for Notch signaling in CRC, VEGF-A and IL-6. Targeting these three pathways may help to design new therapeutic strategies aimed at controlling CRC growht and angiogenesis.

Where applicable, experiments conform with Society ethical requirements