Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA094

Poster Communications

P-Coumaric Acid Ameliorates Renal Ischemia-Reperfusion-Induced Acute Lung Injury By Reducing Oxidative Stress

D. Guzel1, A. Tanyeli2

1. Physiology, Faculty of Medicine, Sakarya University,, Sakarya, Turkey. 2. Physiology, Faculty of Medicine, Ataturk University,, Erzurum, Turkey.

Reduction of oxygen distribution produces reactive oxygen radicals (ROS) such as hydrogen peroxide, superoxide anion and causes cellular dysfunction and necrosis. The release of massive ROS causes activation of stress-induced transcription factors and production of proinflammatory and anti-inflammatory molecules. Numerous molecules have been investigated to prevent this oxidative damage. p-Coumaric acid (p-CA), also known as 4-hydroxycinnamic acid, is one of the potential molecules which has high free radical scavenging, anti-inflammatory and antimicrobial activities. To evaluate the role of p-CA in renal ischemia/reperfusion-induced remote lung injury, thirty-two Wistar Albino adult female rats weighing 200-250 grams (12-16 weeks) were used. These rats were randomly divided into 4 equal groups (n=8). The groups were identified as: sham (S), ischemia-reperfusion (IR), IR+ 50 mg/kg p-CA (p-CA 50), IR+100mg/kg p-CA (p-CA 100). Treatment groups were subjected to surgery in the IR group. Rats in the Sham group underwent right dorsal side nephrectomy. Other groups were clamped in the left renal artery after right dorsal side nephrectomy. p-CA was administered intraperitoneally at single dose immediately before reperfusion. Then the clamp was removed and the reperfusion period started for 3 hours. After the applications were completed, total antioxidant level (TAS), total oxidant level (TOS), superoxide dismutase (SOD), myeloperoxidase (MPO) and malondialdehyde (MDA) levels were measured spectrophotometrically. Oxidative stress index (OSI) was measured. Values are means ± SD., compared by ANOVA. In IR, TOS, MDA, MPO and OSI increased in significant level, while TAS and SOD decreased (p<0.001). An increase in TAS and SOD levels and a decrease in TOS, MDA and MPO values was observed in CGA applied groups (p<0.001). Therefore, our findings suggested that the active ingredient p-CA may exert its antioxidant effects in remote lung injury induced by renal ischemia-reperfusion in rats.

Where applicable, experiments conform with Society ethical requirements