Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA096

Poster Communications

Antioxidative Effects Of Chlorogenic Acid on Lung Injury Induced Renal Ischemia-Reperfusion

A. Tanyeli1, D. Guzel2

1. Physiology, Faculty of Medicine, Ataturk University,, Erzurum, Turkey. 2. Physiology, Faculty of Medicine, Sakarya University,, Sakarya, Turkey.

Renal ischemia/reperfusion (IR) injury resulting in acute renal failure is a major clinical problem due to the high mortality rate. Restoration of the blood flow along with the healing process causes paradoxical reperfusion damage while prolonged interruption of blood flow causes ischemic injury. Although the exact cause is unknown in the etiology of this damage, it is believed that oxidative stress and the formation of reactive oxygen play a key role. Renal IR causes local injury to the kidney, while increased pulmonary capillaries cause lung damage characterized by permeability, edema and neutrophil infiltration. Chlorogenic acid (5-O-caffeoylquinic acid, CGA) is a phenolic compound of the hydroxycinnamic acid family. This polyphenol has anti-oxidant, anti-inflammatory, antilipidemic, antidiabetic activities, among other biological properties. To evaluate the role of CGA in renal ischemia/reperfusion-induced acute lung injury, thirty-two Wistar Albino adult female rats were used. These rats were randomly divided into four equal groups (n=8). The groups were identified as: sham (S), IR, IR+5 mg/kg Chlorogenic Acid (CGA5), IR+10mg/kg Chlorogenic Acid (CGA10). Drug groups were subjected to surgery in the IR group. Rats in the Sham group underwent right dorsal side nephrectomy. Other groups were clamped in the left renal artery after right dorsal side nephrectomy. CGA was intraperitoneally applied in two doses 10 minutes before ischemia and reperfusion. After 1 hour of ischemia, 24 hours of reperfusion was applied to the left kidney. After the applications were completed, total antioxidant level (TAS), total oxidant level (TOS), superoxide dismutase (SOD), myeloperoxidase (MPO) and malondialdehyde (MDA) levels were measured spectrophotometrically. Oxidative stress index (OSI) was measured. In comparison to S, IR group's TAS and SOD values decreased, TOS, MDA and MPO values increased. An increase in TAS and SOD levels, and a decrease in TOS, MDA and MPO values was observed in CGA groups. All of these data indicate that CGA has several roles in IR injury due to its antioxidative properties. In this study, effects of CGA on renal IR model were determined by measuring oxidative stress parameters SOD, TAS, TOS, OSI, MDA and MPO levels. This data demonstrates that CGA has protective effect against renal IR-induced oxidative injury and may be tried as a new therapeutic agent in the future.

Where applicable, experiments conform with Society ethical requirements