Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA134

Poster Communications

Antiapoptotic and Anti-Inflammatory Effects of Urapidil Against Ovarian Tissue Damage In Experimental Ischemia-Reperfusion Model In Rats

A. Tanyeli3, D. Guzel1, S. Doganay1, S. Comakli4, E. Polat2

1. Faculty of Medicine, Physiology, Sakarya Universty, Sakarya, Turkey. 2. Faculty of Medicine, Biochemistry, Atatürk University, Erzurum, Turkey. 3. Faculty of Medicine, Physiology, Atatürk University, Erzurum, Turkey. 4. Veterinary Faculty, Pathology, Atatürk University, Erzurum, Turkey.

Background and Objectives: To investigate the effects of urapidil, as an antioxidant and anti-inflammatory agent, on apoptosis and cell death on ischemia-reperfusion (IR) related ovarian injury. Materials and Methods: Wistar Albino female rats (n=40) were divided into five groups: Sham operation (Group I), IR (Group II), IR plus %15 dimethyl sulfoxide (Group III), IR plus urapidil 0.5 mg/kg (Group IV) and IR plus urapidil 5 mg/kg (Group V). Bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion in study groups. Ovarian tissue TNF-α, IL-1β, NF-κB and Caspase-3 were measured using immunohistopathological methods. Results: Histopathological examination showed that the ovarian tissues has normal histologic in Group I. In other treatment groups, interstitial tissue and corpus luteum were found in haemorrhages. In Group II and Group III haemorrhage were observed to be intense and decreased in treatment groups. The mildest haemorrhage was seen in group V. Immunohistochemical staining of TNFα, IL-1β, NFκB, caspase-3 and LC3B immunopositivities were observed in study groups. The most intense immunopositivity for IL-1β and NFκB were found in Group III. The most intense TNFα immunopositivity was found in Group II. IL-1β, NF-κB, and TNFα immunopositivity decreased in the group IV and group V. In the immunohistochemical staining for apoptotic cell death, in Group I, caspase 3 immunopositivity was not observed. The most intense caspase 3 immunopositivity was observed in Group III. It is observed that caspase 3 immunopositivity decreased in Group IV and Group V. In the Group I, immunopositivity wasn't observed in immunohistochemical staining for autophagic cell death of LC3B. It is observed that immunopositivity was intense in Group II and Group III, but LC3B immunopositivity decreased in Group IV and Group V. Conclusions: In conclusion, urapidil reduces I/R induced inflammation, apoptosis and autophagic cell death in a dose-dependent manner. Our results are promising in the prevention of IR ovarian tissue damage.

Where applicable, experiments conform with Society ethical requirements