Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA215

Poster Communications

The effects of polyphenols supplementation in feed on redox balance parameters in testes of male rats exposed to diesel exhaust

M. Oczkowski1, J. Wilczak2, K. Dziendzikowska1, M. Gajewska2, T. Krolikowski1, J. Gromadzka-Ostrowska1, R. Mruk3, J. Øvrevik4, O. Myhre5, P. Magnusson4, A. Wegierek-Ciuk6, H. Lisowska6, M. Kruszewski7,8,9, A. Lankoff7,6

1. Faculty of Human Nutrition and Consumer Sciences, Department of Dietetics, Warsaw University of Life Sciences, Warsaw, Poland. 2. Faculty of Veterinary Medicine, Department of Physiological Sciences, Warsaw University of Life Sciences, Warsaw, Poland. 3. Faculty of Production Engineering, Department of Production Organization and Engineering, Warsaw University of Life Sciences, Warsaw, Poland. 4. Department of Air Pollution and Noise, Division of Infectious Disease Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway. 5. Department of Toxicology and Risk Assessment, Division of Infectious Disease Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway. 6. Institute of Biology, Department of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland. 7. Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Warsaw, Poland. 8. Department of Medical Biology and Translational Research, University of Information Technology and Management, Rzeszow, Poland. 9. Department of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland.

Exposure to diesel exhaust emission (DEE) is an important source of ambient air pollution with negative health outcomes in humans. Studies demonstrate that exposure to emission from diesel engines increases oxidative stress in the reproductive system (RS). Little is known, whether feed supplementation with polyphenols could reverse the negative impact of DEE on antioxidative defense and oxidative stress parameters in RS. The adult male Fisher 344 rats (308±14g; n=28) were exposed to diesel fuel exhaust (diluted to 2% in air) in the whole body inhalation chambers (exposure: 6h/day, 5 days/week, 4 weeks). The rats were divided into 2 main groups depending on the presence (F+, n=14) or absence (F-, n=14) diesel particle filter (DPF) application. Next, the rats from F+ and F- groups were divided into 2 dietary subgroups and were fed AIN-93 diet supplemented (F+/b, F-/b, n=7) or not (F+/0, F-/0, n=7) with lyophilized black currant marc (80 mg of total polyphenols/100 g feed). After 28 days the rats were anesthetized (by 1-minute inhalation of Isoflurane). The experimental procedures were approved by the 3rd Local Animal Care and Use Committee in Warsaw, Poland. Antioxidant defense indices (total antioxidant status, TAS; superoxide dismutase, SOD; glutathione reductase GR; peroxidase, GPx; and catalase, CAT activities; 2 forms of glutathione, GSH, GSSG), and the levels of oxidative stress markers (lipid hydroperoxides, LPO; 25-hydroxycholesterol, 25-HC, and 7-ketocholesterol, 7-KC) were assessed in testes homogenates. All the values are presented as means ± S.E.M., compared by post hoc Fisher LSD test (ANOVA). The results showed that rats from F-/b group had higher TAS concentration, SOD and GPx activites, and lower GR activity and LPO concentration in testis compared with F-/0 group (TAS: 1.352±0.032 vs. 1.170±0.044 μmol g-1, p<0.001; SOD: 0.301±0.014 U g-1 vs. 0.271±0.009 U g-1; p<0.05; GPx: 0.171±0.034 U g-1 vs. 0.118±0.005; p<0.05; GR: 0.350±0.028 U g-1 vs. 0.551±0.019 U g-1; p<0.01; LPO: 231.5±7.5 vs. 282.9±6.2, p<0.001). Higher SOD and lower GR, LPO, 7-KC and GSSG values were observed in F+/b versus F+/0 group (SOD: 0.348±0.056 U g-1 vs. 0.224±0.006; p<0.01; GR: 0.558±0.030 vs. 0.637±0.007, p<0.05; LPO: 244.3±4.1 vs. 273.3±5.3, p<0.01; 7-KC: 13.0±0.6 vs. 15.8±0.3, p<0.001; GSSG: 2.33±0.07 vs. 2.98±0.25, p<0.05). Catalase (CAT) activity, GSH, and 25-HC concentrations in testis did not significantly differ between groups. The results suggest that exposure to DEE regardless of DPF application disturbs the antioxidant defense balance in rats testes. Intake of black currant cause protecting effects on testes by increasing the anti-oxidative defense and attenuate oxidative stress.

Where applicable, experiments conform with Society ethical requirements