Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA235

Poster Communications

Protective Effects of Magnesium Chloride on Liver Enzymes and Oxidative Stress Biomarkers in Cholesterol diet fed Rats

K. A. MUHAMMED1, A. Abdulrazak1, M. YUSUF1, T. Yusuf2, M. ALIYU2, M. ABDULMALIK2


The excessive consumption of high cholesterol diet has been associated with an increased incidence of obesity. This is because obesity induced pathologies with high mortality, such as complications of dyslipidaemia, diabetes mellitus, arthritis, hypertension, myocardial infarction,and hepatocellular carcinoma. Although the associated, disease are enhanced by formation of oxidative stress, lipid peroxidation and hypercholesterolaemia. Magnesium chloride is found to be beneficial in a wide range of diseases. Magnesium is one of the most neglected mineral in human body. It is crucial for a healthy and lasting life. Magnesium is responsible for the activation of more than 300 enzymes in the body. The present study intends to determine the protective effect of magnesium chloride on liver enzyme and oxidative stress biomarkers in cholesterol diet fed rats. Twenty (20) adult male Wistar rats weighing (180 - 200) grams randomly divided into three treatment and one control groups of five rats each (n = 5). Group I Normal control receive normal feed only for 6weeks, Group II received high fat diet only for 6weeks, Group III received high fat diet with 250 mg/kg for 6weeks of mgcl2 and Group IV received 500 mg/kg for 6weeks of mgcl2 respectively all treatments were administered via oral route, and at the end of the sixth week rats were euthanized and blood samples were drawn from the heart by cardiac puncture and used to estimate oxidative stress biomakers (Superoxide dismutase, Catalase and Gluthation peroxidase) and lipid peroxidation biomarkers (Malondealdehyde), and liver enzymes. Analysis of variance (ANOVA) and Turkey's post hoc test were used to analyze the data obtained. For the oxidative stress biomarkers assessed, the results showed that there was significant decrease (P < 0.05) in the SOD, CAT and GPx level of the HFD groups co-administered with mgcl2 when compared with the HFD fed group only. Also the lipid peroxidation shows significant (p<0.05) decrease in the value of MDA administered with mgcl2 and HFD when compared with HFD group only. For the liver enzyme, The result showed that there was statistical significant (p<0.05) decrease in value of AST, ALT and ALP in the group co-administered with mgcl2 and high fat diet for both 250 mg/kg and 500mg/kg mgcl2, when compared to the HFD group only. The result showed that high-fat diet induces ROS, dyslipidaemia and release of biological metabolite, as evidenced by the rise in oxidative stress and activities of liver enzymes. Mgcl2 increase compensatory mechanisms by inhibiting the rise in biological metabolites in groups treated with it. Mgcl2 administration also protected the body against rise in the metabolites despite consumption of high-fat diet by the Wister Rats.

Where applicable, experiments conform with Society ethical requirements