Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA237

Poster Communications

Does Irisin Affect the Hypothalamic Regulation of Feeding Behavior in High-Fat-Diet-Induced Obese Rats

S. Sandal1, S. Tekin1, Y. Erden2, A. Caglayan3, F. Ozyalin4, C. Colak5, Y. Cigremis6

1. Department of Physiology, Inonu University Medical School, Malatya, Turkey. 2. Department of Molecular Biology and Genetics, Bartin University Faculty of Science, Bartin, Turkey. 3. Department of Physiology, Istanbul Medipol University Medical School, Istanbul, Turkey. 4. Department of Medical Biyochemistry, Inonu University Medical School, Malatya, Turkey. 5. Department of Biostatistic and Medical Informatics, Inonu University Medical School, Malatya, Turkey. 6. Department of Medical Biology and Genetics, Inonu University Medical School, Malatya, Turkey.

The hypothalamus is a key brain region in the regulation of food intake where many factors such as leptin and ghrelin are involved in this control mechanism. Irisin, a novel myokine-derived hormone, has been suggested to play an important role in regulation of feeding behavior and development of obesity. This study aimed to determine the role of irisin on the central regulation of feeding behavior in high-fat-diet-induced obese rats. Sixty-three adults male Wistar-albino rats were used in the study. The rats were randomly divided into 7 groups (n=9) and fed with high-fat-diet-chow (HFDC) for 12 weeks. After development of obesity was confirmed, 10 and 100 nM concentrations of irisin were infused to animals (except control and sham groups) as intracerebroventricularly[1] or intraperitoneally for 14 days. The mRNA gene expression/protein levels of AgRP and CART in the hypothalamus and serum leptin and ghrelin levels were determined. Neither intracerebroventricularly nor intraperitoneally administration of the two different concentrations of irisin in animal fed with HFDC caused any change in body weights, serum leptin and ghrelin levels while irisin caused significant decreases in hypothalamic AgRP and CART gene expression/protein levels in all experiment groups (p<0.05). Our results suggested that irisin can exert a central modulatory role on regulation of feeding behavior and thereby can provide to remain constant of body weight.

Where applicable, experiments conform with Society ethical requirements