Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA242

Poster Communications

Effect of Mesenchymal Stem Cells vs. Nigella Sativa Oil on Streptozotocin Induced Type 1 Diabetes in Rats

S. Mohamed1, E. Ali1, S. Hosny1

1. Histology, Genetics & Cell Biology, Suez Canal University, Ismailia, Egypt.

Diabetes is a chronic metabolic disorder with no definitive cure. Mesenchymal Stem Cells (MSCs) and Nigella Sativa Oil (NSO) are two promising treatments. This study compares the MSCs vs. NSO on streptozotocin (STZ) induced type1 diabetes (T1D) in rat model. Adult female albino rats were divided into 7 equal groups of 10: Control group (G1) received saline, Diabetic groups (G2.1 & G2.2) sacrificed after 15 & 30 days of T1D induction respectively, MSCs treated group 1 (G3.1& G3.2) received IV injection of 1×106 cells/rat 3 days after T1D induction and sacrificed as previous, NSO treated group1 (G4.1 & G4.2) received daily I.P injection of 8 ml/Kg of NSO 3 days after T1D induction and sacrificed as previous. T1D was induced using multiple low doses of STZ (40 mg/Kg). MSCs were isolated from human cord blood samples. Pancreas was extracted and processed for histological and immunohistochemical (IHC) assessment. The mean total islet cell count, percentage of damaged cells, mean number and diameter of islets, and optical density of collagen and brown stained cytoplasm of immunostained sections were calculated using image analysis program. Human male SRY gene sequence in pancreas of female rats was detected by PCR. Values are means ± SEM, compared by ANOVA. Results showed low blood glucose levels in both MSCs and NSO groups, which reached near normal levels within 15 days. Histological assessment of control group showed normal architecture, mean number of islets was 13.6±3.3, mean diameter of islets was 88.7 ±17.2, mean total islet cell count of 2000.70± 90.19 and mean optical density (MOD) of collagen was 0.25 ±0.07. IHC showed strong brown staining of β-cell cytoplasm with MOD of 0.60 ±0.05. G2.1 group showed necrotic changes in islet cells, congested dilated blood capillaries with lymphocytic infiltration. Mean number and diameter of islets and mean total islet cell count were lower compared to control (10.9± 2.6, 66.3± 12.3 & 1017.90± 76.63 respectively). Mean percentage of damaged cells was 52.17 ± 6.62. MOD of collagen was comparable to control, but there was significant decrease in the MOD of the immunoreaction (0.30 ±0.03). The G2.2 group was worse than G2.1. MSCs group (G3.1) showed marked improvement compared to diabetic groups. Mean number and diameter of islets and mean total islet cell count increased (11.0± 1.8, 71.9± 15.4 & 1036.80± 65.44 respectively), while mean percentage of damaged cells decreased (17.16 ± 3.27). MOD of the immunreaction significantly increased compared to diabetic group (0.52 ±0.05). Group G3.2 was better than G3.1. Comparable results were obtained for NSO groups (G4.1). Positive results for SRY gene were detected in MSCs group, indicating homing of stem cells into the pancreas of the female rats. In conclusion, MSCs and NSO exert comparable antidiabetic effects on STZ induced T1D.

Where applicable, experiments conform with Society ethical requirements