Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA243

Poster Communications

Effect of L-Arginine and D-ribose L-cysteine supplementation on stress hormones in 4-vinylcyclohexene diepoxide perimenopausal female rats following acute restraint stress

A. P. Arikawe1, A. W. Olusanya2, O. A. Akinnibosun1, I. C. Udenze3

1. Physiology, College of Medicine, University of Lagos, Lagos, Lagos, Nigeria. 2. Pharmacology, Therapeutics and Toxicology, College of Medicine, University of Lagos, Lagos, Lagos, Nigeria. 3. Clinical Pathology, College of Medicine, University of Lagos, Lagos, Lagos, Nigeria.


Perimenopause involves dynamic neuroendocrine reactions that promote exacerbation of stress responses. L-Arginine and D-ribose have been observed to generally reduce basal levels of stress hormones, but their benefits during perimenopause have not been elucidated. Immature female Sprague-Dawley rats postnatal day 28 from the colony in the animal facilities of the College of Medicine, University of Lagos, Idi-Araba campus were housed in groups of six per cage and were divided into 3 major groups; (1) Control (injected subcutaneously) with Corn oil (2.5 μl/g BW) for 15 days; (2) VCD, (injected subcutaneously) with 4-vinylcyclohexene diepoxide (160 mg/kg BW) from Sigma-Aldrich, United Kingdom diluted in Corn oil (2.5 μl/g BW) for 15 days; and (3) Aged group was left to age naturally till 150 days. Twelve weeks after VCD/corn oil administrations, and 150 days in Aged group, rats were further divided into 3 sub-groups: L-Arginine (administered orally, 100mg/kg BW) from Now Foods, Bloomingdale, USA., D-ribose (administered orally, 200mg/kg BW) from Max international Salt Lake, USA and a third sub-group received neither for additional 30 days. At 135 - 145 days of age in Control and VCD groups., 175 - 185 days of age in Aged group on diestrus morning, animals were subjected to acute restraint stress for 30 minutes. The animals were immediately anaesthetized with tribromoethanol (2, 2, 2-tribromoethanol 99%, Sigma Aldrich, 1 mL/100 g body weight of 2.5% solution, intraperitoneal route) humanely sacrificed. Blood samples were drawn before and after stress from the retro-orbital sinus for measurement of serum prolactin, LH, FSH, progesterone, and corticosterone. In unstressed and stressed animals, L-Arginine and D-ribose significantly increased (p < 0.05) progesterone and corticosterone concentrations in Aged and VCD groups compared to Control group. D-ribose significantly increased (p < 0.05) FSH, LH, and Prolactin concentrations in Aged and VCD groups compared to Control group. L-Arginine significantly decreased (p < 0.05) FSH and LH concentrations in Aged and VCD groups compared to Control group. L-Arginine significantly increased (p < 0.05) prolactin concentrations in unstressed animals and also significantly decreased (p < 0.05) it in stressed animals in the Aged and VCD groups compared to Control group.

Where applicable, experiments conform with Society ethical requirements