Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA244

Poster Communications

PROTECTIVE ROLE OF Allium cepa Linn JUICE ON MATERNAL DEXAMTHASONE INDUCED ALTERATIONS IN REPRODUCTIVE FUNCTION DURING LACTATION IN MALE OFFSPRING OF WISTAR RATS

S. O. Jeje1, L. O. Adegbite2, O. O. Akindele2, O. T. Kunle-Alabi2, Y. Raji2

1. Department of Physiology, Federal University of Technology, AKure, Akure, Ondo State, Nigeria. 2. Department of Physiology, University of Ibadan, Ibadan, Oyo State, Nigeria.


Maternal exposure to dexamethasone during lactation as been reported to increase testicular oxidative stress in the offspring. Allium cepa Linn. (Red Onion) is a known free radical scavenger with antioxidant properties. In this study, the effects of Allium cepa Linn on testicular oxidative stress and disruption of reproductive indices in the male offspring following maternal dexamethasone exposure during lactation in Wistar rats were examined. Twenty (20) lactating dams were assigned into four groups (n=5) and they were treated as follows during lactation: Control (distilled water), dexamethasone (60 µg/kg), Allium cepa Linn (5 ml/kg) and dexamethasone (60 µg/kg)+ Allium cepa Linn (5 ml/kg) . All administration was done orally. Testicular descent, pubertal age were assessed using standard methods. Sperm parameters (Microscopy techniques), hormonal profile (ELISA techniques, Dialab kit Austria), testicular MDA (Buege and Aust, 1978), SOD (Misra and Fridovich, 1972), Catalase (Sinah, 1971) were evaluated. Values (Mean±SEM, n=5) were compared using one way ANOVA. P<0.05 was significant. Serum testosterone (1.69±0.175ng/mL), FSH (0.62±0.040 mIU/mL), LH (0.12± 0.006 mIU/mL) were significantly lower (P<0.05) in the Dex only group when compared with the control (6.49±0.171 ng/mL, 1.79±0.098 mIU/mL, 0.16±0.011 mIU/mL respectively). Sperm count (2.18± 0.370 X 106/ml), motility (32±3.742 %), viability (22± 3.742 %) were significantly lower in the Dex only group when compared with the control (6.84± 1.345 106/ml, 66.00± 4.000%, 76.00± 4.000% respectively). Testicular SOD (998.10±16.730 U/L), Catalase (5.86±0.630 µmol/min/mL), GPx (-3.54±0.522 U/L) were significantly lower relative to the control (2124.00±33.680 U/L, 9.93±0.306 µmol/min/mL, 2.28±0.105 U/L respectively). However, there was a significant increase (P<0.05) in days of testes descent (20.20 ± 0.200 days), preputial separation (69.80 ± 0.200 days ), testicular MDA (43.40 ± 0.143 µM ) with respect to the control(17.60 ± 0.245, days 62.80 ± 0.200 days, 14.30 ± 0.171 µM). Conversely, treatment with Allium cepa Linn. juice significantly (P<0.05) raised serum level of testosterone (4.40 ± 0.153 ng/mL), FSH (0.72 ± 0.045 mIU/mL) and LH (0.14 ± 0.006 mIU/mL), sperm motility (70.00 ±4.472 %), viability (60.00±4.470 %), count (5.62 ±0.396 X 106/ml), testicular SOD (1707 ±22.7U/L), Catalase (8.21 ±1.110 µmol/min/mL ) and GPx (4.93 ±0.422 U/L) of pups in dexamethasone treated rats. Also, Allium cepa Linn signicantly reduced (P<0.05) testicular MDA level (26.46 ±0.099 µM) in the pups of dexamethasone treated dams. The findings from this study suggest that Allium cepa Linn could protect against testicular oxidative stress and disruption of the reproductive axis induced by maternal dexamethasone exposure during lactation.

Where applicable, experiments conform with Society ethical requirements