Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA303

Poster Communications

Study of the effects of the intracerebroventricular and the intrathecal administration of kaempferol and GABAA antagonists on pain in male rats

M. Zarei1, S. Jabbari2, M. Bananej2, A. Komaki1, R. Hajikhani2

1. Department of Physiology, School of Medicine, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran., Hamadan, Iran (the Islamic Republic of). 2. Department of Biology, Faculty of Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran., Tehran, Iran (the Islamic Republic of).

Kaempferol, a type of flavonoid, has antinociceptive activity. The purpose of this research was to evaluate the interaction between the analgesic effects of kaempferol and GABAA receptor in central nervous system in male rat. In this study, we used 220 Wistar male rats. The animals were divided into 22 groups for the intracerebroventricular/ the intrathecal administration. The groups were for each of the two types of administrations: control, DMSO, sham, bicuculline (1 µg), morphine (10 µg), kaempferol (0.5, 1 and 1.5 mg), bicuculline plus kaempferol (0.5, 1 and 1.5 mg). Subjects were anesthetized by Xylazine (10 mg/kg) and Ketamine (100 mg/kg) and placed in the stereotaxic apparatus (Stoelting, USA) for surgery. Pain assessment test was tail flick. All research and animal care procedures were approved by the Veterinary Ethics Committee of the Hamadan University of Medical Science and were performed in accordance with the National Institutes of Health Guide for Care and Use of Laboratory Animals (NIH Publication No. 85-23, revised 1985). Totally, in the tail flick test the dosages of 0.5, 1, 1.5 mg of Kaemferol and Bicuculline plus a dosage of 1 mg of Kaemferol showed noteworthy analgesic effects (p < 0.001). In the intracerebroventricular injection, pain was significantly decreased with the administration of Bicuculline plus the dosages of 0.5 and 1.5 mg of Kaemferol (p < 0.01). However, in the intrathecal administration of Bicuculline plus the dosage of 0.5 mg of Kaemferol attenuated pain of thermal in comparison with groups of control, DMSO, sham, and bicuculline (p < 0.01). The results of this examination showed that Kaemferol has analgesic effects. The modulation of GABAergic system by GABAA receptor antagonist could be involved in this antinociceptive effects.

Where applicable, experiments conform with Society ethical requirements