Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA354

Poster Communications

The effects of Coenzyme Q-10 on gestational diabetes myometrium

S. Kerdsuknirund1, S. Ongsricharoenbhorn1, P. Kupittayanant2, S. Kupittayanant1

1. Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand. 2. Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

Coenzyme Q-10 (CoQ-10 or Ubiquinone) is a naturally occurring quinone that is found in most aerobic organisms from bacteria to mammals. CoQ-10 is believed to be an important cellular antioxidant defense, higher levels of CoQ-10 in gestational diabetes patients may be a compensatory mechanism, in response to an activated oxidative stress, probably associated to hyperglycaemia and insulin resistance (Giannubilo et al., 2011). CoQ-10 has undergone extensive research, but not in the area of smooth muscle physiology. We therefore studied the effects of CoQ-10 on gestational diabetes myometrium. Pregnant rats were randomly distributed into 2 groups: non-gestational diabetic rats and gestational diabetic rats. Induction of diabetes were performed by a single dose of streptozotocin 60 mg/kg body weight with intraperitoneal injection on day 5 of gestation. Blood glucose levels were monitored 2 days after diabetic induction and blood glucose levels equal or higher than 200-300 mg/dL was considered as diabetes. Animals were sacrificed by CO2 asphyxia around 19-21 days of gestation. The uterine horn was removed and longitudinal muscle strips were separated. The strips were attached to a transducer in the organ bath apparatus and changes in isometric force responded to CoQ-10 were measured. QoQ-10 significantly increased myometrial contractile activity (p<0.05). Thus, the integral area under the contraction was increased to 128.29 ± 20.94 in non-gestational diabetic rats and 114.16 ± 15.91 in gestational diabetic rats compared to spontaneous control 100% (n = 5 for each). Although the underlying mechanism whereby QoQ-10 affected the spontaneous uterine contractility in both non-gestational and gestational diabetic rats is not yet clarified, the safety of use as supplementation is concerned.

Where applicable, experiments conform with Society ethical requirements