Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA356

Poster Communications

Effects of pomegranate extract on postpartum sow reproduction

P. Kupittayanant1

1. Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

Pomegranate, Punica granatum L. (Punicaceae), is a nutrient dense fruit rich in phytochemical compounds especially phytoestrogens (Miguel et al., 2010). Extracts of all parts of the pomegranate fruit exhibit therapeutic properties (Lansky and Newman, 2007) and target a range of diseases including infertility. The effects pomegranate extract on female reproduction has never been investigated in the domestic animals. The aim of this work was therefore, to examine the effects of pomegranate extract on sow reproduction. The effects on vaginal cornification and estrous cycle were particularly investigated. To do so, pomegranate fruits were collected, aired dried, and extracted with 95% ethyl alcohol. The extracts were evaporated to dryness under reduced pressure by rotary evaporator, lyophilized by freeze dryer and stored at -20°C until use. Postpartum sows were randomly divided into 3 groups each group contained 4 animals. The animals were orally administrated with distilled water 2 cc/day (group 1), pomegranate extract 0.5 g in 2 cc distilled water/day (group 2), or pomegranate extract 1.0 g in 2 cc distilled water/day (group 3). The administration was started from day 1 after parturition (day 0) until estrus returns (24-27 days). Vaginal smears were checked every week for cornified cells and the day estrus return recorded. The results showed that pomegranate extract helped to shorten the length of estrous cycle in sows. It was shown that the estrous cycle length after parturition was significantly reduced in the groups that received pomegranate extract when compared with the control group (p<0.05). The estrous cycle length was 26.75±0.5, 25.5±0.57, and 25±0.81 in group 1, 2, and 3, respectively. It should be noted that changes in estrus return was consistent with the significant increases in vaginal cornification (p<0.05). The percentage of cornified cells was 80.00±4.08, 82.50±4.78, and 87.50±2.50 in group 1, 2, and 3, respectively. In conclusion, pomegranate extract may help to promote uterine involution leading to shorten the length of estrous cycle in postpartum sows.

Where applicable, experiments conform with Society ethical requirements