Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCA360

Poster Communications

Investigation Effects of Metformin in rats urinary bladder

A. YARDIMCI1, S. Sahinturk2, N. Ulker1, I. Serhatlioglu3, E. Kacar1

1. Department of Physiology, Firat University, ELAZIG, Turkey. 2. Department of Physiology, Uludag University, Bursa, Turkey. 3. Department of Biophysics, Firat University, ELAZIG, Turkey.

A. Yardimci1, S. Sahinturk2, N. Ulker1, I. Serhatlioglu3, E. Kacar1 1. Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey 2. Department of Physiology, Faculty of Medicine, Uludag University, Bursa, Turkey 3. Department of Biophysics, Faculty of Medicine, Firat University, Elazig, Turkey Metformin is currently considered as first medication for the pharmacological management of patients with type 2 diabetes mellitus (T2DM) according to the American Diabetes Association/European Association for Study of Diabetes guidelines. Metformin acts by lowering intestinal glucose absorption, developing peripheral glucose uptake, decreasing fasting plasma insulin levels and boosting insulin sensitivity. Metformin has few adverse side effects, the most widespread is gastrointestinal symptoms, but effects of metformin on urinary bladder is hardly known. In this study, we investigated effects of metformin on rats urinary bladder. Adult female Sprague-Dawley rats (n=12) were used in this study. For examination of the functional role of metformin in bladder, the bladder was quickly removed and placed in Krebs solution and it was formed a flat sheet. Then 12 longitudinal strips were formed from sheet, measuring 2-4 × 6-12 mm and anchored in (5, 10 and 20 mL) organ baths containing Krebs solution and bubbled with 95% O2/ 5 % CO2 at 37°C. The tension formed by the tissues was measured using transducers MP150 instrument (Biopac Systems, Inc., U.S.A.). All tissues was then left to equilibrate for 60 min with rinsing every 30 min under a resting tension of 1.5 g before starting experiment. After equilibration, acetylcholine (10-4 M) was given to the all baths. After one hour rinsing, metformin (2 mM) was given and incubated 30 min. After 30 min, again acetylcholine was given same dose without rinsing and then these part was repeated with metformin 10 mM and 20 mM doses. Control group values were accepted as 100 %, and change calculated as percent (%) for all treatment groups for peak to peak amplitude, frequency and area values. Data were given as means ± SE and statistical analysis of the results was implemented by one way ANOVA. In all conditions, differences were considered significant when p<0.05. 2 mM dose has no significant effects compared to control at frequency and area values, but it has significant increase at peak to peak amplitude (127.65±4.24, change %, p<0.01). Also, peak to peak amplitude has significantly increased in 10 mM and 20 mM (163.86±8.57 and 162.58±6.76, respectively, change %, p<0.001) compared to control. Frequency has significantly decreased in 10 mM (37.75±4.71, change %, p<0.001) and 20 mM (16.29±2.41 change %, p<0.001). In addition, area has significantly increased at only 20 mM dose (3767.64±733.70 change %, p<0.001) compared to control. In conclusion, metformin increased urinary bladder contraction in dose-dependent manner.

Where applicable, experiments conform with Society ethical requirements