Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB008

Poster Communications

Increased DNA damage with comet assay in patients with pulmonary hypertension

E. Kilic-Toprak2, Y. Yaylali1, Y. Ozdemir2, V. Kucukatay2, H. Senol3, M. Bor-Kucukatay2

1. Department of Cardiology, Pamukkale University Faculty of Medicine, Denizli, Turkey. 2. Department of Physiology, Pamukkale University Faculty of Medicine, Denizli, Turkey. 3. Department of Biostatistic, Pamukkale University Faculty of Medicine, Denizli, Turkey.

  • DNA damage assay parameters of the study groups<\#13>

Pathologic lesions involve increased oxidative stress and inflammatory processes in pulmonary hypertension (PH) (1). The purpose of our study was to investigate the potential contribution of DNA damage in PH. The study comprised 28 untreated PH patients (59.93±11.19 years) and 28 age- and sex-matched (59.86±11.92 years) healthy controls. All participants underwent a right-heart catheterization to confirm the diagnosis and to asses hemodynamics. Fifty cells per slide/sample were scored to evaluate DNA damage using the comet assay (2). Several parameters including head length, tail length, head intensity, tail intensity, tail moment, tail migration were evaluated. Continuous data were reported as mean ± standard deviation. Mann Whitney U test was used for independent groups. For categorical variables, Chi-square test was used. SPSS, 24.0 was used for statistical analyses and p value less than 0.05 was considered statistically significant. The average pulmonary vascular resistance was 5.64±2.99 woods, mean right atrial pressure 9.70±5.38 mmHg, cardiac index 3.12±1.18 l/min/m2, mixed venous O2 saturation was 64.77±13.33. In our study, PH patients had significantly higher tail length, tail moment and tail migration values compared to the control group (Table 1). Higher tail length values are known as the signs of increased DNA breaks, however tail moment, which is derived from the tail length and % DNA in the tail, shows more stable predicting performance for DNA damage according to most researchers. These results suggest that patients with PH could have increased DNA damage and this damage could be contributing to the pathophysiology of the disease

Where applicable, experiments conform with Society ethical requirements