Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB019

Poster Communications

Effect of Nifedipine on Heart Glucose 6-Phosphate Dehydrogenase of Ischemia / Reperfusion Injured Rat

A. Tanyeli1, E. Senturk2, E. Eraslan3, M. Güler1, M. Sentürk4

1. Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey. 2. Physiology, Agri Ibrahim Cecen University, School of Health Services,, Ağri, Turkey. 3. Physiology, Bozok University, Faculty of Medicine, Yozgat, Turkey. 4. Biochemistry, Agri Ibrahim Cecen University, Pharmacy Faculty, Ağri, Turkey.

AIM: The purpose of this study is to investigate the effect of nifedipine drug on rat heart tissue glucose 6-phosphate dehydrogenase (G6PD) enzyme in ischemia / reperfusion (I / R) and I / R injured rats. METHODS: Control, sham, I / R (1:24 hours) and nifedipine + I / R are as follows: A total of 24 male Wistar albino rats weighing 250-300 g were equally and randomly divided into four groups. In the last group, nifedipine was administered at the 4 mg/kg dose before intraperitoneal ischemia period. Heart tissues were removed after bilateral I/R process. Tissue levels of enzyme activity of glucose 6-phosphate dehydrogenase (G6PD) were measured. Enzymatic activity was measured by using Beutler's method by using a spectrophotometer. The assay system contained 0.1 mM Tris-HCl (pH 8.5) with 0.5 mM EDTA, 10 mM MgCl2, 0.2 mM NADP+ and 0.6 mM (G6P) in a total volume of 1 ml. RESULTS: Specific activity values were determined for G6PD enzyme at four different experimental groups. Groups were determined as follows: Control group 0.109 EU/mg protein, sham group 0.082 EU/mg protein, I/R group 0.114 EU/mg protein, and nifedipine + I/R group 0.079 EU/mg protein. CONCLUSION: As a result, the activity of the G6PD enzyme was determined to be mostly inhibited in nifedipine + I/R group among all applications. Activity value of nifedipine + I/R group decreased by about 28% compared to controls was observed that p ≤ 0.05 is as meaningful.

Where applicable, experiments conform with Society ethical requirements