Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB072

Poster Communications

Anti-oxidant and Anti-inflammatory effects of Melatonin on TBARS, GSH-Px, SOD, and YKL-40 in Endotoxemic Lung Tissue of Wistar albino Rats

E. Ozkok1, H. Yorulmaz2, G. Ates Ulucay3,4, V. Olgac5, S. Tamer4

1. Department of Neuroscience, Istanbul University, Aziz Sancar Institute of Experimental Medicine, Istanbul, Turkey. 2. Department of Physiology, Halic University, Faculty of Medicine, Istanbul, Turkey. 3. Department of Physiology, Istanbul Yeni Yuzyil University, Faculty of Medicine, Istanbul, Turkey. 4. Department of Physiology, Istanbul University, Istanbul Medical Faculty, Istanbul, Turkey. 5. Department of Pathology, Istanbul University,, Istanbul, Turkey.

Motivation/problem statement: In the literature, there are studies that melatonin has protective effects on oxidative stress and excessive cytokines release (1). However, there are a few study about relation with lung tissue damage using high and multi doses of melatonin during endotoxemia (2). Lipopolysaccharide (LPS) as bacterial endotoxin, is used agent for forming experimentally endotoxemia (3). YKL-40 is known carbohydrate binding protein, a member of chitinase family, that have been shown degrade and remodelling of extracellular matrix in endotelial cells. YKL-40 identified by proteomic analysis is considered as a biomarker of sepsis. (4). Oxidative stress is occur the imbalance between antioxidants defence mechanisms and increased free radical species in cells (5). In the organism, Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px), and Glutathione reductase (GR), are an important antioxidant enzymes, scavenges reactive radicals that occured in oxidative stress conditions in chronic diseases such as sepsis, inflammatory diseases (6). We aimed to investigate the effects of melatonin on inflammatory and oxidative stress parameters and tissue histology of Wistar albino male rats endotoxemia induced by LPS. Methods/procedure/approach: We seperated male Wistar albino rats (210-230g) to 4 groups as; control, LPS (10 mg / kg i.p.), Melatonin (3 * 10 mg / kg i.p.), Melatonin + LPS. Six hours after the first injection, all animals were sacrificed taking blood from the heart under anesthesia for histological and biochemical parameters. Lung tissues were removed at -80 °C for ELISA and TBARS analyzes, and in 10% formaldehyde for histological examinations. Results: TBARS values were significantly (p <0.05) higher both in the melatonin + LPS and LPS groups than that of control group (p <0.01). Glucose, Alanine Amino Tranferase and Aspartate Aminotransferase enzymes values were significantly higher in the LPS group than those of control group (p <0.01). It was observed compared other groups, GSH-Px (p<0.01) and YKL-40 (p<0.05) were decreased, and both of levels of GR and SOD (p<0.05) were increased in the LPS group. In the histological investigation, control and Melatonin group's lung tissues were observed to be in normal structure. In the LPS group, it has been found that alveoli in the lung tissue are destroyed in several areas, in which the septum is thickened and contains inflammatory cells infiltration. In the LPS+Melatonin group, the indications in the LPS group were delayed, but the findings were still continuing in a few areas. Conclusion/implications: According to our results, we proposed that melatonin's has improving effect on the inflammatory and oxidant-antioxidant systems in endotoxemic rats.

Where applicable, experiments conform with Society ethical requirements