Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB081

Poster Communications

On Application of Cardiac Preparations of Fish In Physiological Education and Research

E. Neu1, M. C. Michailov1, A. Werner-Srivastava1,2, D. G. Weiss1,3, G. Weber1,4, G. Werner1,5

1. Institute Umweltmedizin c/o ICSD/IAS e.V. POB 340316, 80100 M. (Int. Council Sci. Develop./Int. Acad. Sci. Berlin-Bratislava-Innsbruck-Muenchen-NewDelhi-Paris-Sofia-Vienna), Muenchen, Germany. 2. Sana Klinikum Offenbach, Gynecology, Offenbach, Germany. 3. University Rostock, Institute Cell Physiol. (Dir.a.D.), Rostock, Germany. 4. University Lxbg. & Vienna, Faculty Psychol. (Dean), Vienna, Austria. 5. Klinikum Darmstadt, Clinic Internal Med. I (Dir.), Darmstadt, Germany.

INTRODUCTION In medical education&research are till today usual cardio-vascular preparations of frog, rat, rabbit, etc. An essential part of experiments with isolated angio-cardiac preparations could be replaced by fish organs. About pharmaco-physiological reactions of fish heart of salmo gairdner is reported [1,3]. Now is given information about ionic-dependent thermo-induced reactions: These demonstrate some common action mechanisms with mechanosensitive ionic channels of vesical (detrusor, trigone) myocytes [2]. METHOD Isolated heart and vascular strips from rainbow-trout (about 300g) from fish-shop are investigated about elementary physiological properties incl. motor actvity recording) & influence of physiological factors in Krebs-Henseleit-solution [1]. RESULTS Atrium&vantriculus carotis generate spontaneous contractions: [Ca++]-increase has positive, [K+] negative chrono- and inotropic effects. Negative acetylcholine and positive adrenaline effects (10nM-1µM) are antagonized by atropine&propranolol resp. (1µM). Ouabain (strophantine, 0.1-1µM) induce strong augmentation of cardiac contractions. Thermo-induced effects are strong dependent on ionic concentration. Change of some pharmaov-physiological factors (hormones, drugs) induce different rhythmic burst-like motor pattern at 14-24°C, contrary to regular&uniform motor activity at 6°C. CONCLUSION Fish cardiac preparations could reduce enormous application of animal organs in pharmacological industry and education&research at universities, i.e. this could laed to large animal protection. Table Thermo-induced reactions in trout atrium in dependence of CaCl2 and KCl At 24°C Atrium contraction frequency 67±23/min=100%, amplitudes 35±51 mm=100%, i.e. 4.7% of the prep.length of 0.6±1.3mm Isotonic recording, mean±standard deviation, p<0.05<0.01 A. CaCl2 2.1mmol/l=1x contr. ampl. in % of initial value (n=18) 24°C, 0.25x: 78±60, 1x: 213±118, 2x: 271±170 6°C, 0.25x: 24±16, 1x: 89±75, 2x: 156±152 contr. freq./min in % of initial value 24°C, 0.25x: 106±62, 1x: 87±47, 2x: 52±35 6°C, 0.25x: 28±15, 1x: 35±16, 2x: 31±14 B. KCl 4.6mmol/l=1x contr. ampl. in % of initial value (n=18) 24°C, 0.25x: 149±70, 1x: 113±65, 2x: 43±24 6°C, 0.25x: 118±120, 1x: 69±61, 2x: 3±6 contr. freq./min in % of initial value 24°C, 0.25x: 106±60, 1x: 97±93, 2x: 71±73 6°C, 0.25x: 34±14, 1x: 24±13, 2x: 2±4

Where applicable, experiments conform with Society ethical requirements